15/30311173 DC : 0

Superseded

BS EN ISO 15216-1 - MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS IN FOOD USING REAL-TIME RT-PCR - PART 1: METHOD FOR QUANTIFICATION

Available format(s)

Hardcopy , PDF

Superseded date

30-04-2017

Language(s)

English

Publisher

British Standards Institution

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus and materials
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Precision
12 Test report
Annex A (normative) - Diagram of procedure
Annex B (informative) - Real-time RT-PCR
        mastermixes and cycling parameters
Annex C (informative) - Real-time RT-PCR primers
        and hydrolysis probes for the detection of
        HAV, norovirus GI and GII and mengo
        virus (process control)
Annex D (informative) - Growth of mengo virus
        strain MC[0] for use as a process control
Annex E (informative) - RNA extraction using
        the BioMerieux NucliSens[R] system
Annex F (normative) - Composition and preparation
        of reagents and buffers
Annex G (informative) - Generation of double-stranded
        DNA (dsDNA) control stocks
Annex H (informative) - Generation of external control
        RNA (EC RNA) stocks
Annex I (informative) - Typical optical plate layout
Annex J (informative) - Results of interlaboratory study
Bibliography

BS EN ISO 15216-1.

Committee	AW/9
DocumentType	Draft
Pages	54
PublisherName	British Standards Institution
Status	Superseded
SupersededBy	BS EN ISO 15216-1:2017

ISO 22174:2005	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 22118:2011	Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics
ISO 22119:2011	Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions