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    AS 4276.1-1995

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    Water microbiology General information and procedures

    Available format(s):  Hardcopy, PDF 1 User, PDF 3 Users, PDF 5 Users, PDF 9 Users

    Superseded date:  16-09-2023

    Language(s): 

    Published date:  05-03-1995

    Publisher:  Standards Australia

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    Table of Contents - (Show below) - (Hide below)

    1 - AS 4276.1-1995 WATER MICROBIOLOGY - GENERAL INFORMATION AND PROCEDURES
    1 - PREFACE
    1 - METHOD
    1 - 1 SCOPE
    1 - 2 REFERENCED DOCUMENTS
    2 - 3 TYPES OF WATER
    2 - 4 USE OF THE METHODS
    2 - 5 GOOD LABORATORY PRACTICE
    3 - 6 SAMPLING AND SAMPLES
    3 - 6.1 General
    3 - 6.2 Storage of samples
    3 - 7 GLASSWARE
    3 - 8 CLEANING OF NEW GLASSWARE, RUBBERWARE, AND NON-STERILE PLASTICS
    3 - 8.1 New borosilicate glass, rubberware, and non-sterile plastics
    3 - 8.2 New soda glass
    4 - 9 CLEANING OF USED LABORATORY EQUIPMENT
    4 - 9.1 Preliminary treatment
    4 - 9.2 Use of washing machines
    4 - 9.3 Test tubes, Petri dishes, flasks and bottles
    4 - 9.4 Pipettes
    4 - 9.5 Rubber stoppers and sealing liners
    4 - 9.6 Caps
    5 - 10 STERILIZATION OF EQUIPMENT
    5 - 10.1 General
    5 - 10.2 Oven method
    5 - 10.3 Autoclave method
    5 - 11 MEDIA, DILUENTS AND REAGENTS
    6 - 12 REFERENCE CULTURES
    6 - 13 INCUBATION TEMPERATURES
    6 - 14 DILUTIONS
    6 - 14.1 General
    6 - 14.2 Preparation of dilutions
    6 - 15 GENERAL PROCEDURE FOR ESTIMATING THE MOST PROBABLE NUMBER (MPN) OF MICROORGANISMS
    6 - 15.1 Principle
    6 - 15.2 Diluents and culture media
    6 - 15.3 Apparatus
    7 - 15.4 Preparation of dilutions and inoculation of medium
    7 - 15.4.1 General
    7 - 15.4.2 Procedure
    7 - 15.5 Incubation
    7 - 15.6 Calculation
    9 - 16 GENERAL PROCEDURE FOR COLONY COUNTS USING MEMBRANE FILTRATION
    9 - 16.1 Principle
    9 - 16.2 Diluents and culture media
    9 - 16.3 Apparatus
    11 - 16.4 Preparation of apparatus and materials
    11 - 16.4.1 Sterilization
    11 - 16.4.2 Medium
    11 - 16.5 Filtration procedure
    11 - 16.5.1 General
    11 - 16.5.2 Procedure
    12 - 16.6 Incubation
    12 - 16.7 Staining, counting and identification
    12 - 16.8 Calculation
    13 - 17 REFERENCES

    Abstract - (Show below) - (Hide below)

    This Standard sets out general information on the microbiological examination of water using the methods in this series.

    Scope - (Show below) - (Hide below)

    This Standard sets out general information on the microbiological examination of water using the methods in this series.

    General Product Information - (Show below) - (Hide below)

    Committee FT-020
    Document Type Standard
    Publisher Standards Australia
    Status Superseded
    Superseded By
    Supersedes
    Under Revision

    History - (Show below) - (Hide below)

    First published as part of AS 1095.4.1.1-1981.Revised and redesignated in part as AS 4276.1-1995. First published as part of AS 1095.4.1.1-1981. Revised and redesignated in part as AS 4276.1-1995. First published as part of AS 1095.4.1.1-1981. Revised and redesignated in part as AS 4276.1-1995.

    Standards Referenced By This Book - (Show below) - (Hide below)

    AS 2182-1998 Sterilizers - Steam - Benchtop
    AS 2252.2-1994 Biological safety cabinets - Laminar flow biological safety cabinets (Class II) for personnel, environment and product protection
    AS 2192-1991 Sterilizers - Steam - Downward displacement
    AS 2031.2-1987 Selection of containers and preservation of water samples for chemical and microbiological analysis - Microbiological
    AS 2252.1-1994 Biological safety cabinets - Biological safety cabinets (Class I) for personnel and environment protection

    Standards Referencing This Book - (Show below) - (Hide below)

    AS/NZS 4276.19:2001 Water microbiology Examination for thermophilic spp.- Membrane filtration
    AS 4276.11-1995 Water microbiology - Pseudomonads Membrane filtration method
    AS 4276.13-1995 Water microbiology Pseudomonas aeruginosa - Membrane filtration method
    AS 4276.21-2005 Water microbiology Examination for coliforms and Escherichia coli - Determination of most probable number (MPN) using enzyme hydrolysable substrates
    AS/NZS 4276.17.1:2000 Water microbiology Spores of sulfite-reducing anaerobes (clostridia) including filtration method
    AS/NZS 4276.3.1:2007 Water microbiology Heterotrophic colony count methods - Pour plate method using yeast extract agar
    AS 4276.3.1-1995 Water microbiology Heterotrophic colony count methods - Pour plate method using plate count agar
    AS 4276.6-1995 Water microbiology Thermotolerant coliforms and Escherichia coli - Estimation of most probable number (MPN)
    AS/NZS 4276.20:2003 Water microbiology Examination for coagulase positive staphylococci, including Staphylococcus aureus, by membrane filtration
    AS 4276.4-1995 Water microbiology Coliforms - Estimation of most probable number (MPN)
    AS/NZS 4276.18:2001 Water microbiology - Aeromonas by membrane filtration including selected speciation
    AS 4276.5-1995 Water microbiology Coliforms - Membrane filtration method
    AS 4276.14-1995 Water microbiology Salmonellae
    AS/NZS 4276.17.2:2000 Water microbiology Spores of sulfite-reducing anaerobes (clostridia) including - Estimation of most probable number (MPN) using the multiple tube dilution technique
    AS 4276.9-1995 Water microbiology Faecal streptococci - Membrane filtration method
    AS 4276.7-1995 Water microbiology Thermotolerant coliforms and Escherichia coli - Membrane filtration method
    AS/NZS 4276.3.2:2003 Water microbiology Heterotrophic colony count methods - Plate count of water containing biocides (Reconfirmed 2013)
    AS 4276.10-1995 Water microbiology Pseudomonads - Estimation of most probable number (MPN)
    AS 4276.12-1995 Water microbiology Pseudomonas aeruginosa - Estimation of most probable number (MPN)
    AS 4276.8-1995 Water microbiology Faecal streptococci - Estimation of most probable number (MPN)
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