1 - AS 4276.21-2005 WATER MICROBIOLOGY - EXAMINATION FOR COLIFORMS AND ESCHERICHIA COLI-DETERMINATION OF MOST PROBABLE...
1 - PREFACE
1 - FOREWORD
2 - METHOD
2 - 1 SCOPE
2 - 2 REFERENCED DOCUMENTS
2 - 3 DEFINITIONS AND ABBREVIATIONS
2 - 3.1 Definitions
2 - 3.1.1 Coliforms
2 - 3.1.2 Escherichia coli
2 - 3.2 Abbreviations
2 - 4 PRINCIPLE
3 - 5 CULTURE MEDIA AND REAGENTS (see Appendix B)
3 - 5.1 Culture media
3 - 5.1.1 EHS medium with ONPG and MUG
3 - 5.2 Reagents
3 - 5.2.1 Buffered peptone water
3 - 5.2.2 Peptone solution, 0.1 percent
3 - 5.2.3 Ringers solution, quarter strength
3 - 6 APPARATUS
3 - 6.1 Multiple tube dilution technique (MPN) (Refer to AS 4276.4)
3 - 6.1.1 Rimless bacteriological test tubes or equivalent of appropriate size.
3 - 6.1.2 Containers
3 - 6.1.3 Pipettes or equivalent measuring devices
3 - 6.2 Multiple well technique
3 - 6.2.1 Pre-sterilized 100 mL containers
3 - 6.2.2 Pre-sterilized multiple well trays
3 - 6.2.3 Multiple well tray sealer
3 - 6.3 Common apparatus
3 - 6.3.1 Incubators or water baths
3 - 6.3.2 Colour/fluorescence comparators
3 - 6.3.3 Ultra violet viewing cabinet
3 - 7 REFERENCE CULTURES
3 - 7.1 Positive cultures
3 - 7.1.1 Enterobacter aerogenes
4 - 7.1.2 Escherichia coli
4 - 7.2 Negative cultures
4 - 7.2.1 Aeromonas hydrophila
4 - 7.3 Use of reference cultures
4 - 8 SAMPLES
4 - 8.1 Laboratory samples
4 - 8.2 Dilutions
4 - 9 PROCEDURE
4 - 9.1 Multiple tube dilution technique (Refer to AS 4276.1)
5 - 9.2 Multiple well technique
5 - 10 INTERPRETATION OF THE TESTS
6 - 11 DETERMINATION OF MOST PROBABLE NUMBER (MPN) OF COLIFORMS AND E. coli
6 - 12 TEST REPORT
7 - APPENDIX A - FLOW CHART FOR COLIFORMS AND E. coli BY THE ENZYME HYDROLYSABLE SUBSTRATE (EHS) METHOD
8 - APPENDIX B - CULTURE MEDIA AND REAGENTS
8 - B1 SCOPE
8 - ¿B2 ENZYME HYDROLYSABLE SUBSTRATE WITH O-NITROPHENYL-(-D-GALACTOPYRANOSIDE ¿ONPG) AND 4METHYLUMBELLIFERYL-¿-D...
8 - B3 BUFFERED PEPTONE WATER
9 - B4 PEPTONE SOLUTION, 0.1 PERCENT
9 - B5 RINGERS SOLUTION, FULL STRENGTH
10 - APPENDIX C - PREPARATION OF A COMPARATOR
10 - C1 INTRODUCTION
10 - C2 PROCEDURE