Water microbiology Water quality - Enumeration of Escherichia coli and coliform bacteria - Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014/Amd 1:2016, MOD)

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English

Published date

07-03-2019

Publisher

Standards Australia

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The objective of this Standard is to specify a method for the enumeration of Escherichia coli (E. coli) and coliform bacteria.

This part of ISO 9308 specifies a method for the enumeration of Escherichia coli (E. coli) and coliform bacteria. The method is based on membrane filtration, subsequent culture on a chromogenic coliform agar medium, and calculation of the number of target organisms in the sample. Due to the low selectivity of the differential agar medium, background growth can interfere with the reliable enumeration of E. coli and coliform bacteria, for example, in surface waters or shallow well waters. This method is not suitable for these types of water.
This part of ISO 9308 is especially suitable for waters with low bacterial numbers that will cause less
than 100 total colonies on chromogenic coliform agar (CCA). These may be drinking water, disinfected pool water, or finished water from drinking water treatment plants.
Some strains of E. coli which are ß-D-glucuronidase negative, such as Escherichia coli O157, will not be
detected as E. coli. As they are ß-D-galactosidase positive, they will appear as coliform bacteria on this chromogenic agar.

Committee	FT-020
DocumentType	Standard
ISBN	978 1 76072 395 8
Pages	16
ProductNote	Modifies ISO 9308-1:2014 + AMD1:2016.
PublisherName	Standards Australia
Status	Current
Supersedes	AS/NZS 4276.22:2007 DR AS 4276.22:2018

First published as AS/NZS 4276.22:2007.
Revised and redesignated as AS 4276.22:2019. First published as AS/NZS 4276.22:2007. Revised and redesignated as AS 4276.22:2019.

ISO 19458:2006	Water quality Sampling for microbiological analysis
ISO 11133:2014	Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
ISO 3696:1987	Water for analytical laboratory use — Specification and test methods
ISO 8199:2018	Water quality — General requirements and guidance for microbiological examinations by culture
ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 7704:1985	Water quality — Evaluation of membrane filters used for microbiological analyses

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AS 4276.22:2019

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