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  • AS/NZS 2243.3:1995

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    Safety in laboratories Microbiology

    Available format(s):  Hardcopy, PDF 1 User, PDF 3 Users, PDF 5 Users, PDF 9 Users

    Superseded date:  30-06-2017

    Language(s): 

    Published date:  05-06-1995

    Publisher:  Standards Australia

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    Table of Contents - (Show below) - (Hide below)

    1 - AS/NZS 2243.3:1995 SAFETY IN LABORATORIES - MICROBIOLOGY
    4 - PREFACE
    5 - CONTENTS
    8 - FOREWORD
    9 - SECTION 1 SCOPE AND GENERAL
    9 - 1.1 SCOPE
    9 - 1.2 REFERENCED DOCUMENTS
    10 - 1.3 DEFINITIONS
    10 - 1.3.1 Aerosol
    10 - 1.3.2 Antiseptic
    10 - 1.3.3 Aseptic technique
    10 - 1.3.4 Biological containment
    10 - 1.3.5 Cross-contamination
    10 - 1.3.6 Decontamination
    10 - 1.3.7 Disinfectant
    11 - 1.3.8 Exposure standard
    11 - 1.3.9 Flexible film isolator
    11 - 1.3.10 Genetic Manipulation Advisory Committee (GMAC)
    11 - 1.3.11 HEPA filter
    11 - 1.3.12 Infectious
    11 - 1.3.13 Institutional biosafety committee (IBC)
    11 - 1.3.14 Microbiological hazard
    11 - 1.3.15 Pathogen
    11 - 1.3.16 Physical containment
    11 - 1.3.17 Primary barrier
    11 - 1.3.18 Secondary barrier
    12 - 1.3.19 Sharps
    12 - 1.3.20 Sterilization
    12 - 1.3.21 Viable
    13 - SECTION 2 ORGANIZATION AND RESPONSIBILITY
    13 - 2.1 RESPONSIBILITY
    13 - 2.1.1 Management policy
    13 - 2.1.2 Management responsibility
    13 - 2.1.3 Safety officer responsible for microbiology
    13 - 2.1.4 Laboratory supervisor
    13 - 2.1.5 Personal responsibility and behaviour
    14 - 2.2 HEALTH MANAGEMENT
    14 - 2.2.1 Injuries
    14 - 2.2.2 Blood samples
    14 - 2.2.3 Immunization
    14 - 2.2.4 High risk persons
    14 - 2.2.5 Antenatal precautions
    14 - 2.3 PROVISION OF SERVICES
    14 - 2.4 IMPORTED BIOLOGICAL MATERIALS
    15 - 2.5 INCIDENT/INJURY REPORTING
    15 - 2.6 FIRST AID KIT
    16 - SECTION 3 CLASSIFICATION OF LABORATORIES, PRACTICES AND PROCEDURES
    16 - 3.1 GENERAL
    16 - 3.2 CLASSIFICATION OF LABORATORIES
    16 - 3.2.1 General
    16 - 3.2.2 Physical Containment Level 1 (PC1)
    17 - 3.2.3 Physical Containment Level 2 (PC 2)
    17 - 3.2.4 Physical Containment Level 3 (PC3)
    17 - 3.2.5 Physical Containment Level 4 (PC4)
    17 - 3.3 LABORATORIES FOR GENETIC MANIPULATION
    17 - 3.4 PHYSICAL CONTAINMENT LEVEL 1 (PC1) REQUIREMENTS
    17 - 3.4.1 General
    18 - 3.4.2 Laboratory facilities
    18 - 3.4.3 Personal protective clothing and equipment
    18 - 3.4.4 Work practices
    19 - 3.5 PHYSICAL CONTAINMENT LEVEL 2 (PC2) REQUIREMENTS
    19 - 3.5.1 General
    20 - 3.5.2 Laboratory facilities
    20 - 3.5.3 Personal protective equipment
    20 - 3.5.4 Containment equipment
    21 - 3.5.5 Work practices
    22 - 3.6 PHYSICAL CONTAINMENT LEVEL 3 (PC3) REQUIREMENTS
    22 - 3.6.1 General
    22 - 3.6.2 Laboratory facilities
    23 - 3.6.3 Laboratory ventilation
    23 - 3.6.4 Personal protective equipment
    23 - 3.6.5 Containment equipment
    23 - 3.6.6 Work practices
    24 - 3.6.7 Health monitoring
    24 - 3.7 PHYSICAL CONTAINMENT LEVEL 4 (PC4) REQUIREMENTS
    24 - 3.7.1 General
    24 - 3.7.2 Laboratory facilities
    25 - 3.7.3 Personal protective equipment
    25 - 3.7.4 Containment equipment
    25 - 3.7.5 Work practices
    26 - 3.7.6 Health monitoring
    27 - 3.7.7 Positive pressure suit area
    27 - 3.8 GUIDELINES FOR LARGE SCALE GENETIC MANIPULATION WORK
    28 - SECTION 4 DEGREE OF HAZARD FROM MICROORGANISMS
    28 - 4.1 CLASSIFICATION OF INFECTIVE MICROORGANISMS BY RISK GROUP
    28 - 4.2 RISK-GROUPING OF MICROORGANISMS BY TYPE
    28 - 4.2.1 General
    28 - 4.2.2 Bacteria, Chlamydiae, Rickettsiae and Mycoplasmas
    28 - 4.2.3 Parasites
    28 - 4.2.4 Fungi
    29 - 4.2.5 Viruses
    29 - 4.2.6 Exotic animal viruses
    29 - 4.2.7 Fish pathogens
    29 - 4.2.8 Plant pathogens
    39 - SECTION 5 LABORATORY SPILLS
    39 - 5.1 GENERAL
    39 - 5.2 PLANNING
    39 - 5.3 SPILLS INSIDE BIOLOGICAL SAFETY CABINETS
    40 - 5.4 SPILLS OUTSIDE BIOLOGICAL SAFETY CABINETS
    40 - 5.4.1 General
    40 - 5.4.2 Spills of human blood and body fluids
    40 - 5.4.3 Spills of minor risk
    40 - 5.4.4 Spills of major hazard
    41 - 5.5 CENTRIFUGE SPILLS
    42 - SECTION 6 GENERAL PRECAUTIONS AND SPECIAL EQUIPMENT
    42 - 6.1 GENERAL PRECAUTIONS
    42 - 6.1.1 Toxic substances
    42 - 6.1.2 Cleaning
    42 - 6.1.3 Discard containers
    42 - 6.1.4 Bunsen burners
    42 - 6.1.5 Disposable syringes and needles
    42 - 6.2 CENTRIFUGES
    42 - 6.2.1 General
    42 - 6.2.2 Sealed-bucket and sealed-rotor centrifuges
    43 - 6.2.3 Other centrifuges
    43 - 6.3 FREEZE-DRYING AND RECONSTITUTION OF CULTURES
    43 - 6.4 LIQUID NITROGEN
    44 - 6.5 PRESSURE STEAM STERILIZERS
    44 - 6.5.1 General
    44 - 6.5.2 Air removal
    44 - 6.5.3 Downward displacement sterilizers
    45 - 6.5.4 Pre-vacuum sterilization
    45 - 6.5.5 Times for sterilization
    45 - 6.5.6 Monitoring of sterilization cycles
    45 - 6.6 BIOLOGICAL SAFETY CABINETS
    46 - 6.7 CYTOTOXIC DRUG SAFETY CABINETS
    47 - SECTION 7 WASTE DISPOSAL
    47 - 7.1 COLLECTION
    47 - 7.2 TREATMENT AND DISPOSAL OF WASTES
    48 - SECTION 8 TRANSPORT OF INFECTIOUS AND OTHER BIOLOGICAL MATERIALS
    48 - 8.1 GENERAL
    48 - 8.2 TRANSPORT REGULATIONS
    48 - 8.3 IMPORTATION REGULATIONS
    48 - 8.4 CATEGORIES OF BIOLOGICAL MATERIALS
    49 - 8.5 DOCUMENTATION
    52 - SECTION 9 LABORATORY CLEANING
    52 - 9.1 GENERAL
    52 - 9.2 CLEANING PERSONNEL
    52 - 9.3 BENCHES AND WORK SURFACES
    52 - 9.4 WALLS AND SHELVES
    52 - 9.5 FLOOR CLEANING
    53 - SECTION 10 WORK AREAS
    53 - 10.1 WARNING SIGNS
    53 - 10.2 SEGREGATION OF SPECIAL AREAS
    53 - 10.3 ANIMAL AREAS
    54 - SECTION 11 ANIMALS AND ANIMAL HOUSES
    54 - 11.1 GENERAL
    54 - 11.2 PERSONAL SAFETY
    54 - 11.3 DISSECTION AND POST-MORTEM EXAMINATIONS
    54 - 11.4 STERILIZATION AND WASTE DISPOSAL
    54 - 11.5 PRELIMINARY PROCEDURES FOR GENETIC MANIPULATION WORK
    54 - 11.5.1 General
    55 - 11.5.2 Principal investigator
    55 - 11.5.3 Institutional Biosafety Committee
    55 - 11.6 REQUIREMENTS FOR HOUSING OF INFECTIOUS ANIMALS
    56 - 11.7 DESIGN OF ANIMAL HOUSE FACILITIES
    56 - 11.7.1 Animal housing
    56 - 11.7.2 Details of animal involved
    56 - 11.7.3 Type of work
    56 - 11.7.4 Details of the genetic manipulation
    57 - 11.7.5 Other work being done in the same holding facility
    57 - 11.7.6 Disposal of animals
    57 - 11.7.7 Transport of animals
    57 - 11.8 REQUIREMENTS FOR ANIMAL HOUSE CONTAINMENT LEVEL PC2
    57 - 11.8.1 Construction
    57 - 11.8.2 Operating procedures
    58 - 11.9 REQUIREMENTS FOR ANIMAL HOUSE CONTAINMENT LEVEL PC3
    58 - 11.9.1 General
    59 - 11.9.2 Construction
    59 - 11.9.3 Operating procedures
    61 - SECTION 12 CLASSIFICATION OF PLANT HOUSES, PRACTICES AND PROCEDURES
    61 - 12.1 GENERAL
    61 - 12.2 REQUIREMENTS FOR PLANT HOUSE LEVEL PC1
    61 - 12.2.1 General
    61 - 12.2.2 Construction
    61 - 12.2.3 Operating procedures
    61 - 12.3 REQUIREMENTS FOR PLANT HOUSE LEVEL PC2
    61 - 12.3.1 General
    61 - 12.3.2 Construction
    61 - 12.3.3 Operating procedures
    62 - 12.4 REQUIREMENTS FOR PLANT HOUSE LEVEL PC3
    62 - 12.4.1 General
    62 - 12.4.2 Construction
    63 - 12.4.3 Operating procedures
    63 - 12.5 REQUIREMENTS FOR PLANT HOUSE LEVEL PC4
    63 - 12.5.1 General
    63 - 12.5.2 Construction
    63 - 12.5.3 Operating procedures
    64 - SECTION 13 CLASSIFICATION OF INSECT LABORATORIES, PRACTICES AND PROCEDURES
    64 - 13.1 REQUIREMENTS FOR INSECT HOUSE LEVEL PC2
    64 - 13.1.2 Construction
    64 - 13.1.3 Operating procedures
    65 - 13.2 REQUIREMENTS FOR INSECT HOUSE LEVEL PC3
    65 - 13.2.1 General
    65 - 13.2.2 Construction
    65 - 13.2.3 Operating procedures
    65 - 13.3 REQUIREMENTS FOR INSECT HOUSE LEVEL PC4
    65 - 13.3.1 General
    66 - 13.3.2 Construction
    66 - 13.3.3 Operating procedures
    67 - SECTION 14 BIBLIOGRAPHY
    68 - APPENDIX A - TYPICAL MICROBIOLOGICAL INCIDENT/ILLNESS REPORT
    69 - APPENDIX B - CHEMICAL DISINFECTANTS
    69 - B1 INTRODUCTION
    69 - B2 SUSCEPTIBILITY OF MICROORGANISMS
    69 - B3 TYPES OF CHEMICAL DISINFECTANTS
    69 - B4 FACTORS AFFECTING ACTIVITY OF DISINFECTANTS
    69 - B5 CHOICE OF DISINFECTANT
    70 - B6 PROPERTIES OF COMMONLY-USED DISINFECTANTS
    70 - B6.1 Chlorine
    70 - B6.2 Iodine
    70 - B6.3 Formaldehyde
    71 - B6.4 Glutaraldehyde
    72 - B6.5 Peracetic acid
    72 - B6.6 Peroxygen biocides
    72 - B6.7 Hydrogen peroxide
    72 - B6.8 Alcohols
    72 - B6.9 Phenolics
    72 - B6.10 Quaternary ammonium compounds
    73 - B6.11 Chlorhexidine
    73 - B6.12 Acids and alkalis
    73 - B7 CONTAMINATION OF DISINFECTANTS
    75 - APPENDIX C - BIOLOGICAL HAZARD SYMBOL
    76 - APPENDIX D - REFERENCES AND RELATED DOCUMENTS
    76 - D1 REFERENCES
    77 - D2 RELATED DOCUMENTS

    Abstract - (Show below) - (Hide below)

    Specifies requirements and gives recommended practices relating to safety in laboratories in which microorganisms are handled. Includes descriptions of risk group categories for microorganisms and corresponding containment level facilities.

    Scope - (Show below) - (Hide below)

    This Standard sets out requirements, responsibilities and general guidelines relating to safety in laboratories where microorganisms are handled. It is intended for laboratories, including animal and plant houses, where microbiological work such as research, teaching, diagnosis, quality control and regulatory analysis, e.g. of foodstuffs, water and effluents, pharmaceuticals and cosmetics, is undertaken. This Standard should be read in conjunction with AS 2243.1.

    General Product Information - (Show below) - (Hide below)

    Committee CH-026
    Document Type Standard
    Publisher Standards Australia
    Status Superseded
    Superseded By
    Supersedes

    History - (Show below) - (Hide below)

    Amendment 2 to AS/NZS 2243.3:1995 see DR 97356 CP. Under Revision see DR 00254 First published as AS 2243.3-1979.Second edition 1985.Third edition 1991.Jointly revised and designated AS/NZS 2243.3:1995. First published as AS 2243.3-1979. Second edition 1985. Third edition 1991. Jointly revised and designated AS/NZS 2243.3:1995.

    Standards Referenced By This Book - (Show below) - (Hide below)

    AS 2639-1994 Laminar flow cytotoxic drug safety cabinets - Installation and use
    AS/NZS 1715:1994 Selection, use and maintenance of respiratory protective devices
    AS 1324-1973 Air filters for use in air conditioning and general ventilation
    AS 1336-1982 Recommended practices for eye protection in the industrial environment
    AS 2243.2-1990 Safety in laboratories - Chemical aspects
    AS 1807.6-1989 Cleanrooms, workstations and safety cabinets - Methods of test - Determination of integrity of terminally mounted HEPA filter installations
    AS 4260.1(INT)-1994 High efficiency particulate air (HEPA) filters Performance and construction
    AS 2647-1994 Biological safety cabinets - Installation and use
    AS 2567-1994 Laminar flow cytotoxic drug safety cabinets
    AS 1319-1994 Safety signs for the occupational environment (Reconfirmed 2018)
    AS 2982-1987 Laboratory construction
    AS 2252.2-1994 Biological safety cabinets - Laminar flow biological safety cabinets (Class II) for personnel, environment and product protection
    AS 1410-1987 Sterilizers - Steam - Pre-vacuum
    AS 2252.1-1994 Biological safety cabinets - Biological safety cabinets (Class I) for personnel and environment protection
    AS 2243.8-1992 Safety in laboratories - Fume cupboards
    AS 4031-1992 Non-reusable containers for the collection of sharp medical items used in health care areas
    AS 2243.4(INT)-1994 Safety in laboratories Ionizing radiations
    AS 2243.9-1991 Safety in laboratories - Recirculating fume cabinets
    AS 2508.6.026-1984 Safe storage and handling information card - Formaldehyde solutions - Flashpoint above 61 degrees Celsius
    AS 1132.5-1973 Methods of test for air filters for use in air conditioning and general ventilation - Arrestance efficiency, average arrestance efficiency, dust-holding capacity, and dust-holding capacity per unit of effective face area for test dusts Nos 1, 2 and 3
    AS 2243.1-1990 Safety in laboratories - General
    AS/NZS 1337:1992 Eye protectors for industrial applications
    AS 1885-1976 Code of practice for recording and measuring work injury experience

    Standards Referencing This Book - (Show below) - (Hide below)

    AS 1774.13-2000 Refractories and refractory materials - Physical test methods Permanent dimensional change (Reconfirmed 2013)
    AS/NZS 2243.1:1997 Safety in laboratories - General
    AS/NZS 4020:1999 Products for use in contact with drinking water
    AS/NZS 2869:1998 Tampons - Menstrual
    AS/NZS 4308:2001 Procedures for the collection, detection and quantitation of drugs of abuse in urine
    AS 4439.3-1997 Wastes, sediments and contaminated soils Preparation of leachates - Bottle leaching procedures
    AS/NZS 2647:2000 Biological safety cabinets - Installation and use
    AS/NZS 3816:1998 Management of clinical and related wastes
    AS 1774.22-2000 Refractories and refractory materials - Physical test methods Refractory mortar - Test methods
    AS/NZS 2243.8:2001 Safety in laboratories - Fume cupboards
    AS 4479.2-1997 Analysis of soils Extraction of heavy metals and metalloids from soil by aqua regia - Hotplate digestion method
    AS 4439.2-1997 Wastes, sediments and contaminated soils Preparation of leachates - Zero headspace procedure
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