1 - AS/NZS 4276.6:2007 WATER MICROBIOLOGY - METHOD 6: COLIFORMS, ESCHERICHIA COLI AND THERMOTOLERANT COLIFORMS-DETERMINATION OF M
1 - PREFACE
2 - METHOD
2 - 1 SCOPE
2 - 2 REFERENCED DOCUMENTS
2 - 3 DEFINITIONS
2 - 3.1 Coliforms
2 - 3.2 Thermotolerant coliforms
2 - 3.3 Escherichia coli (E. coli)
2 - 4 PRINCIPLE
3 - 5 CULTURE MEDIA AND REAGENTS (See Appendix B)
3 - 6 APPARATUS
3 - 7 REFERENCE CULTURES
3 - 7.1 Escherichia coli, NCTC 9001 or ATCC 11775 or ACM 1803 or NZRM 3309 or a culture traceable to any of these strains
3 - 7.2 Enterobacter aerogenes, NCTC 10006 or ATCC 13048 or ACM 4982 or NZRM 798 or a culture traceable to any of these strains
3 - 7.3 Pseudomonas aeruginosa, NCTC 10332 or ATCC 10145 or ACM 495 or NZRM 3007 or a culture traceable to any of these strains
3 - 7.4 Use of reference cultures
4 - 8 SAMPLES
4 - 8.1 Laboratory samples
4 - 8.2 Dilutions
4 - 9 PROCEDURE
4 - 9.1 Presumptive test
5 - 9.2 Confirmatory tests
5 - 9.2.1 Coliforms
5 - 9.2.2 E. coli
6 - 9.2.3 Thermotolerant coliforms
6 - 10 DETERMINATION OF THE MOST PROBABLE NUMBER (MPN) OF COLIFORMS, E. COLI AND THERMOTOLERANT COLIFORMS
6 - 11 TEST REPORT
6 - 12 BIBLIOGRAPHY
7 - APPENDIX A - FLOW DIAGRAM FOR COLIFORMS, E. COLI AND THERMOTOLERANT COLIFORMS-MOST PROBABLE NUMBER
8 - APPENDIX B - CULTURE MEDIA AND REAGENTS
8 - B1 SCOPE
8 - B2 IMPROVED FORMATE LACTOSE GLUTAMATE MEDIUM
10 - B3 ORTHO-NITROPHENYL-β D-GALACTOPYRANOSIDE (ONPG) BROTH
10 - B4 NUTRIENT AGAR
11 - B5 MACCONKEY AGAR
11 - B6 OXIDASE REAGENT (KOVACS’)
11 - B7 EC-MUG BROTH
12 - APPENDIX C - PERFORMANCE OF KOVACS’ OXIDASE TEST