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ASTM D 4455 : 1985 : R1997

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure
Available format(s)

Hardcopy , PDF

Superseded date

11-11-2014

Language(s)

English

Published date

10-12-1997

Publisher

American Society for Testing and Materials

CONTAINED IN VOL. 11.02, 2015 Defines a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure.

1.1 This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. It is applicable to environmental waters.

1.2 Certain types of debris and other microorganisms may fluoresce in acridine orange-stained smears.

1.3 The test method requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.

1.4 Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.

1.5 Approximately 1014 cells/mL are required for detection by this test method.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Committee
D 19
DocumentType
Test Method
Pages
3
ProductNote
Reconfirmed 1997
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy

ASTM E 2800 : 2011 : R2017 Standard Practice for Characterization of <emph type="ital">Bacillus</emph> Spore Suspensions for Reference Materials

ASTM D 1129 : 2013 : REDLINE Standard Terminology Relating to Water

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PDF - English
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