Customer Support: 131 242

  • There are no items in your cart
We noticed you’re not on the correct regional site. Switch to our AMERICAS site for the best experience.
Dismiss alert

ASTM D 5712 : 2015 : R2020

Current
Current

The latest, up-to-date edition.

Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified Lowry Method
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

01-06-2020

1.1This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with NR, latex, and elastomeric products. Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances that may interfere with the determination. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.

1.2For the purpose of this test method, the range of protein measurement will be based on the limit of detection and quantitation and recorded in micrograms per dm2 test specimen.

1.3The test method is designed to be accurate and compatible with the industrial environment.

1.4Steps are included in this test method to minimize the effects of interfering substances.

1.5It is recognized that other methods for the analysis of leachable proteins exist and these may be used for routine quality control purposes provided they have been validated and a correlation established against the reference method specified by this test method.

1.6This test method has not been validated for use with lubricated products such as condoms. Condoms with different lubricants as typically marketed, have not been tested in an ASTM ILS to determine if, and if so to what degree, the lubricant interferes with the assay.

1.7This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.8This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Committee
D 11
DocumentType
Test Method
Pages
9
PublisherName
American Society for Testing and Materials
Status
Current
Supersedes

ASTM D 7329 : 2007 : R2018 Standard Specification for Food Preparation and Food Handling (Food Service) Gloves
ASTM D 3578 : 2019 Standard Specification for Rubber Examination Gloves
ASTM D 3577 : 2019 Standard Specification for Rubber Surgical Gloves
ASTM D 7198 : 2005 : R2018 Standard Specification for Disposable Embalming Gloves for Single-Use Applications
ASTM D 6499 : 2018 Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products
ASTM D 7427 : 2016 Standard Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Hevea Natural Rubber and Its Products Derived from Latex
ASTM D 7850 : 2019 Standard Specification for Biological Properties of Industry Reference Materials (IRM)
ASTM D 7103 : 2019 Standard Guide for Assessment of Medical Gloves

ASTM D 3577 : 2019 : R2023 Standard Specification for Rubber Surgical Gloves
ASTM D 3578 : 2019 : R2023 Standard Specification for Rubber Examination Gloves

View more information
$103.13
Including GST where applicable

Access your standards online with a subscription

Features

  • Simple online access to standards, technical information and regulations.

  • Critical updates of standards and customisable alerts and notifications.

  • Multi-user online standards collection: secure, flexible and cost effective.

Need help?
Call us on 131 242, then click here to start a Screen Sharing session
so we can help right away! Learn more