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ASTM D 6499 : 2018

ASTM D 6499 : 2018

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products

Available format(s)

Hardcopy , PDF

Superseded date

13-06-2023

Language(s)

English

Published date

21-08-2018

Publisher

American Society for Testing and Materials

1.1 This test method covers an immunological method todetermine the amount of antigenic protein in Hevea NaturalRubber and its products using rabbit antisera specific forHNRL proteins.

1.1This test method covers an immunological method to determine the amount of antigenic protein in Hevea Natural Rubber and its products using rabbit antisera specific for HNRL proteins. This immunoassay procedure quantitatively measures the level of antigenic latex proteins in solution using an inhibition format. The samples may include glove or other product extracts which have been collected in order to measure the HNR levels. Although this method detects antigenic proteins, it should not be considered as a measure of allergenic proteins. Correlation of protein/antigen levels with the level of allergenic proteins has not been fully established.

1.2For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities.

1.3This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.4This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Committee
D 11
DocumentType
Test Method
Pages
9
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

ASTM D 7427 : 2016 Standard Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Hevea Natural Rubber and Its Products Derived from Latex
ASTM D 7103 : 2018 Standard Guide for Assessment of Medical Gloves
ASTM D 7850 : 2018 Standard Specification for Biological Properties of Industry Reference Materials (IRM)
ASTM D 3578 : 2005 : R2015 Standard Specification for Rubber Examination Gloves
ASTM D 3577 : 2009 : R2015 Standard Specification for Rubber Surgical Gloves
ASTM D 8243 : 2019 Standard Test Method for Determination of APS Reductase to Estimate Sulfate Reducing Bacterial Bioburdens in Water – Enzyme-Linked Immunosorbent Assay Method
ASTM D 1076 : 2015 Standard Specification for Rubber—Concentrated, Ammonia Preserved, Creamed, and Centrifuged Natural Latex
ASTM D 3578 : 2019 Standard Specification for Rubber Examination Gloves
ASTM D 7427 : 2016 : R2021 Standard Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Hevea Natural Rubber and Its Products Derived from Latex (Withdrawn 2023)
ASTM D 3577 : 2019 Standard Specification for Rubber Surgical Gloves
ASTM D 1076 : 2021 Standard Specification for Rubber—Concentrated, Ammonia Stabilized, Creamed, and Centrifuged Natural Latex
ASTM D 7103 : 2019 Standard Guide for Assessment of Medical Gloves
ASTM D 7850 : 2019 Standard Specification for Biological Properties of Industry Reference Materials (IRM)
ASTM D 7198 : 2005 : R2018 Standard Specification for Disposable Embalming Gloves for Single-Use Applications
ASTM D 7329 : 2007 : R2018 Standard Specification for Food Preparation and Food Handling (Food Service) Gloves

ASTM E 177 : 1990 : REV A : R1996 Standard Practice for Use of the Terms Precision and Bias in ASTM Test Methods
ASTM D 5712 : 2015 : R2020 Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified Lowry Method
ASTM D 5712 : 2015 Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified Lowry Method
ASTM D 4483 : 1999 Standard Practice for Determining Precision for Test Method Standards in the Rubber and Carbon Black Industries
ASTM D 4483 : 2020 Standard Practice for Evaluating Precision for Test Method Standards in the Rubber and Carbon Black Manufacturing Industries
ASTM D 4483 : 2005 : REV A Standard Practice for Evaluating Precision for Test Method Standards in the Rubber and Carbon Black Manufacturing Industries
ASTM D 5712 : 2005 Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method
ASTM E 691 : 2020 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM D 5712 : 1999 Standard Test Method for the Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method
ASTM D 5712 : 2010 Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method
ASTM E 691 : 2019 : EDT 1 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM E 691 : 2023 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM D 5712 : 1999 : EDT 1 Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method
ASTM E 691 : 2022 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM E 691 : 2009 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM E 691 : 2021 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM D 4483 : 2005 Standard Practice for Evaluating Precision for Test Method Standards in the Rubber and Carbon Black Manufacturing Industries
ASTM D 4483 : 2014 : REV A Standard Practice for Evaluating Precision for Test Method Standards in the Rubber and Carbon Black Manufacturing Industries
ASTM D 4483 : 2014 Standard Practice for Evaluating Precision for Test Method Standards in the Rubber and Carbon Black Manufacturing Industries
ASTM D 4483 : 2018 Standard Practice for Evaluating Precision for Test Method Standards in the Rubber and Carbon Black Manufacturing Industries
ASTM D 4483 : 2005 : REV A : R2011 Standard Practice for Evaluating Precision for Test Method Standards in the Rubber and Carbon Black Manufacturing Industries
ASTM D 5712 : 2005 : EDT 1 Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method

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