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ASTM E 1440 : 1991 : R2012

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Guide for Acute Toxicity Test with the Rotifer Brachionus (Withdrawn 2021)
Available format(s)

Hardcopy , PDF

Superseded date

09-11-2023

Language(s)

English

Published date

07-01-2013

CONTAINED IN VOL. 11.06, 2013 Gives the procedures for obtaining laboratory data concerning the acute toxicity of chemicals and aqueous effluents releases into fresh, estuarine or marine waters.

1.1This guide describes procedures for obtaining laboratory data concerning the acute toxicity of chemicals and aqueous effluents released into fresh, estuarine, or marine waters. Acute toxicity is measured by exposing Brachionus newly hatched from cysts to a series of toxicant concentrations under controlled conditions. This guide describes a test for using B. calyciflorus, a fresh water rotifer, and the Appendix describes modifications of this test for estuarine and marine waters using B. plicatilis. These procedures lead to an estimation of acute toxicity, including the concentration expected to kill 50 % of the test rotifers (LC50) in 24 h. Procedures not specifically stated in this guide should be conducted in accordance with Guide E729 and Guide E1192.

1.2Modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using modified procedures might not be comparable to rotifer acute tests that follow the protocol described here. Comparison of the results using modified procedures might provide useful information concerning new concepts and procedures for conducting acute toxicity tests on chemicals and aqueous effluents.

1.3This guide is organized as follows:

Section

Scope

1

Referenced Documents

2

Terminology

3

Summary of Guide

4

Significance and Use

5

Apparatus

6

Dilution Water

7

Hazards

8

Test Material

9

Test Organisms

10

Test Procedure

11

Calculation of Results

12

Acceptability of the Test

13

Report

14

Keywords

15


1.4These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or mixtures. This guide can also be used to conduct investigations of the effects on rotifer survival of pH, hardness, and salinity and on materials such as aqueous effluents, leachates, oils, particulate matter, sediments, and surface waters. This guide might not be appropriate for materials with high oxygen demand, with high volatility, subject to rapid biological or chemical transformation, or that readily sorb to test chambers.

1.5This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazards statements, see Section 8.

Committee
E 50
DocumentType
Guide
Pages
8
ProductNote
Reconfirmed 2012
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

ASTM D 6954 : 2018 Standard Guide for Exposing and Testing Plastics that Degrade in the Environment by a Combination of Oxidation and Biodegradation
ASTM E 1850 : 2004 : R2019 Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests
ASTM D 6046 : 2018 Standard Classification of Hydraulic Fluids for Environmental Impact

ASTM E 380 : 1993 Standard Practice for Use of the Internation System of Units (SI) (the Modernized Metric System) (Withdrawn 1997)
ASTM E 1192 : 1997 : R2008 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1192 : 1997 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1192 : 1997 : R2003 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 729 : 1996 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1192 : 1997 : R2014 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians

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