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ASTM E 1706 : 2000 : EDT 1

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Fresh Water Invertebrates
Available format(s)

Hardcopy , PDF

Superseded date

11-11-2014

Language(s)

English

Published date

31-12-2010

1.1 This test method covers procedures for testing freshwater organisms in the laboratory to evaluate the toxicity of contaminants associated with whole sediments. Sediments may be collected from the field or spiked with compounds in the laboratory.

1.1.1 Test methods are described for two toxicity test organisms, the amphipod Hyalella azteca ( H. azteca) (see 13.1.2) and the midge Chironomus tentans (C. tentans) (see 14.1.2). The toxicity tests are conducted for 10 days in 300-mL chambers containing 100 mL of sediment and 175 mL of overlying water. Overlying water is renewed daily and test organisms are fed during the toxicity tests. Endpoints for the 10-day toxicity tests are survival and growth. These test methods describe procedures for testing freshwater sediments; however, estuarine sediments (up to 15 ppt salinity) can also be tested with H. azteca. In addition to the 10-day toxicity test method outlined in 13.1.2 and 14.1.2, general procedures are also described for conducting 10-day sediment toxicity tests with H. azteca (see 13.1.2) and C. tentans(see 14.1.2).

1.1.2 Guidance for conducting sediment toxicity tests is outlined in Annex A1 for Chironomus riparius, in Annex A2 for Daphnia magna and Ceriodaphnia dubia, in Annex A3 for Hexagenia spp., in Annex A4 for Tubifex tubifex, and in for the Diporeia spp. Guidance is also provided in Annex A7 for conducting long-term sediment toxicity tests with H. azteca by measuring effects on survival, growth, and reproduction. Guidance is also provided in for conducting long-term sediment toxicity tests with C. tentans by measuring effects on survival, growth, emergence, and reproduction. outlines the data that will be needed before test methods are developed from the guidance outlined in to for these test organisms. General procedures described in Sections 17 for sediment testing with H. azteca and C. tentans are also applicable for sediment testing with the test organisms described in Annex A1 to Annex a7.

1.2 Procedures outlined in this test method are based primarily on procedures described in the United States Environmental Protection Agency (USEPA) (1-8 ) and Guides E 1367, E 1391, E 1525 and E 1688.

1.3 Additional research and methods development are now in progress to: (1) evaluate additional test organisms, (2) further evaluate the use of formulated sediment, (3) refine sediment dilution procedures, (4) refine sediment toxicity identification evaluation (TIE) procedures (9), (5) refine sediment spiking procedures, (6) develop in situ toxicity tests to assess sediment toxicity and bioaccumulation under field conditions, (7) evaluate relative sensitivities of endpoints measured in tests, (8) develop methods for new species, (9) evaluate relationships between toxicity and bioaccumulation, and (10) produce additional data on confirmation of responses in laboratory tests with natural populations of benthic organisms. Some issues that may be considered in interpretation of test results are the subject of continuing research including the influence of feeding on bioavailability, nutritional requirements of the test organisms, and additional performance criteria for organism health. See Section 6 for additional detail. This information will be described in future editions of this standard.

1.4 The USEPA (1) and Guide E 1688 also describes 28-day bioaccumulation methods for the oligochaete Lumbriculus variegatus.

1.5 Results of tests, even those with the same species, using procedures different from those described in the test method may not be comparable and using these different procedures may alter bioavailability. Comparison of results obtained using modified versions of these procedures might provide useful information concerning new concepts and procedures for conducting sediment tests with aquatic organisms. If tests are conducted with procedures different from those described in this test method, additional tests are required to determine comparability of results. General procedures described in this test method might be useful for conducting tests with other aquatic organisms; however, modifications may be necessary.

Committee
E 47
DocumentType
Test Method
Pages
118
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

ASTM E 1563 : 2021 Standard Guide for Conducting Short-Term Chronic Toxicity Tests with Echinoid Embryos
ASTM E 1688 : 2019 Standard Guide for Determination of the Bioaccumulation of Sediment-Associated Contaminants by Benthic Invertebrates
ASTM E 2186 : 2002 : REV A : R2016 Standard Guide for Determining DNA Single-Strand Damage in Eukaryotic Cells Using the Comet Assay
ASTM E 1688 : 2010 : R2016 Standard Guide for Determination of the Bioaccumulation of Sediment-Associated Contaminants by Benthic Invertebrates
ASTM E 1295 : 2001 : R2013 Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with <emph type="ital">Ceriodaphnia dubia</emph><emph type="bold">
ASTM E 1850 : 2004 : R2019 Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests
ASTM E 1563 : 1998 : R2012 Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos
ASTM E 1611 : 2000 : R2013 Standard Guide for Conducting Sediment Toxicity Tests with Polychaetous Annelids
ASTM E 1676 : 2012 Standard Guide for Conducting Laboratory Soil Toxicity or Bioaccumulation Tests with the Lumbricid Earthworm <i>Eisenia Fetida</i> and the Enchytraeid Potworm <i>Enchytraeus albidus</i>
ASTM E 2552 : 2016 Standard Guide for Assessing the Environmental and Human Health Impacts of New Compounds for Military Use
ASTM E 1391 : 2003 : R2014 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
ASTM E 2172 : 2001 : R2014 Standard Guide for Conducting Laboratory Soil Toxicity Tests with the Nematode <i xmlns:a="http://library.astm.org" xmlns=""> Caenorhabditis elegans</i>
ASTM E 2455 : 2006 : R2013 Standard Guide for Conducting Laboratory Toxicity Tests with Freshwater Mussels (Withdrawn 2022)
ASTM E 724 : 1998 : R2012 Standard Guide for<brk type="line"/> Conducting Static Acute Toxicity Tests Starting with Embryos of Four Species of Saltwater Bivalve Molluscs
ASTM E 1439 : 2012 : R2019 Standard Guide for Conducting the Frog Embryo Teratogenesis Assay-Xenopus (FETAX)
ASTM E 3163 : 2018 Standard Guide for Selection and Application of Analytical Methods and Procedures Used during Sediment Corrective Action
ASTM E 1525 : 2002 : R2014 Standard Guide for Designing Biological Tests with Sediments
ASTM E 1841 : 2004 : R2012 Standard Guide for Conducting Renewal Phytotoxicity Tests With Freshwater Emergent Macrophytes (Withdrawn 2021)
ASTM E 1367 : 2003 : R2014 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM E 2591 : 2007 : R2013 Standard Guide for Conducting Whole Sediment Toxicity Tests with Amphibians
ASTM E 2122 : 2002 : R2013 Standard Guide for Conducting In-situ Field Bioassays With Caged Bivalves (Withdrawn 2022)

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