1.1This test method provides detailed instructions for performing a quantitative evaluation of antimicrobial efficacy of a towelette when challenged against Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella enterica. The method may be used with other microbial strains, though modification may be necessary to accommodate recovery.

1.2This quantitative method does not differentiate between mechanical removal of inoculum from a surface and chemical inactivation of the test microbe; rather, product efficacy is considered a combination of both attributes of a towelette-based formulation.

1.3It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP Standards—40 CFR, Part 160 of FIFRA) are required and to follow them when appropriate.

1.4This standard may involve the use of hazardous materials, chemicals and infectious microorganisms and should be performed only by persons with formal training in microbiology.

1.5Strict adherence to the protocol is necessary for the validity of the test results.

1.6The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7This standard does not address specific product performance standards established by regulatory authorities; see Section 10, Note 2 for details.

1.8This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.