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EN 15204:2006

Current

Current

The latest, up-to-date edition.

Water quality - Guidance standard on the enumeration of phytoplankton using inverted microscopy (Utermöhl technique)

Published date

30-08-2006

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Equipment and preservatives
6 Sample processing
7 Counting procedure
8 Quantitative validation
9 Measurement uncertainty
Annex A (informative) Optical characteristics of inverted
                      microscopes
Annex B (informative) Sample treatment
Annex C (informative) Phytoplankton analysis strategies
Annex D (informative) Identification
Annex E (informative) Use of conventional compound
                      microscopes
Annex F (informative) Statistical procedure
Bibliography

The procedure described in this European Standard is based on the standard settling technique as defined by Utermöhl in 1958 [31]. It describes a general procedure for the estimation of abundance and taxonomic composition of marine and freshwater phytoplankton by using inverted light microscopy and sedimentation chambers, including the preceding steps of preservation and storage. Emphasis is placed on optimizing the procedure for the preparation of the microscopic sample. Many of the general principles of the approach described may also be applied to other techniques of enumerating algae (or other entities) using a (conventional) microscope, some of which are described in Annex E. This guidance standard does not cover field collection of samples or the analysis of picoplankton, quantitative analysis of free-floating mats of Cyanobacteria or specific preparation techniques for diatoms.

Committee
CEN/TC 230
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

Standards Relationship
I.S. EN 15204:2006 Identical
NF EN 15204 : 2006 Identical
SN EN 15204 : 2006 Identical
BS EN 15204:2006 Identical
UNE-EN 15204:2007 Identical
DIN EN 15204 E : 2006 Identical
NEN EN 15204 : 2006 Identical
UNI EN 15204 : 2006 Identical
NBN EN 15204 : 2006 Identical
PN EN 15204 : 2006 Identical
NS EN 15204 : 1ED 2006 Identical
DIN EN 15204:2006-12 Identical

NF EN 15972 : 2011 WATER QUALITY - GUIDANCE ON QUANTITATIVE AND QUALITATIVE INVESTIGATIONS OF MARINE PHYTOPLANKTON
UNE-EN 16698:2016 Water quality - Guidance on quantitative and qualitative sampling of phytoplankton from inland waters
13/30291787 DC : 0 BS EN 16698 - WATER QUALITY - GUIDANCE ON QUANTITATIVE AND QUALITATIVE SAMPLING OF PHYTOPLANKTON FROM INLAND WATERS
09/30203919 DC : 0 BS EN 15972 - WATER QUALITY - GUIDANCE ON QUANTITATIVE AND QUALITATIVE INVESTIGATIONS OF MARINE PHYTOPLANKTON
EN ISO 5667-3:2012 Water quality - Sampling - Part 3: Preservation and handling of water samples (ISO 5667-3:2012)
DIN EN 16695:2015-12 WATER QUALITY - GUIDANCE ON THE ESTIMATION OF PHYTOPLANKTON BIOVOLUME
UNE-EN 15972:2012 Water quality - Guidance on quantitative and qualitative investigations of marine phytoplankton
DIN EN 16698 E : 2015 WATER QUALITY - GUIDANCE ON QUANTITATIVE AND QUALITATIVE SAMPLING OF PHYTOPLANKTON FROM INLAND WATERS
BS EN 16698:2015 Water quality. Guidance on quantitative and qualitative sampling of phytoplankton from inland waters
UNI EN 15708 : 2010 WATER QUALITY - GUIDANCE STANDARD FOR THE SURVEYING, SAMPLING AND LABORATORY ANALYSIS OF PHYTOBENTHOS IN SHALLOW RUNNING WATER
UNE-EN 15708:2010 Water quality - Guidance standard for the surveying, sampling and laboratory analysis of phytobenthos in shallow running water
UNI EN ISO 5667-3 : 2013 WATER QUALITY - SAMPLING - PART 3: PRESERVATION AND HANDLING OF WATER SAMPLES
BS EN 15708:2009 Water quality. Guidance standard for the surveying, sampling and laboratory analysis of phytobenthos in shallow running water
NF EN 15708 : 2010 WATER QUALITY - GUIDANCE STANDARD FOR THE SURVEYING, SAMPLING AND LABORATORY ANALYSIS OF PHYTOBENTHOS IN SHALLOW RUNNING WATER
BS EN 16695:2015 Water quality. Guidance on the estimation of phytoplankton biovolume
DIN EN 16698:2015-12 WATER QUALITY - GUIDANCE ON QUANTITATIVE AND QUALITATIVE SAMPLING OF PHYTOPLANKTON FROM INLAND WATERS
DIN EN 15708 E : 2010 WATER QUALITY - GUIDANCE STANDARD FOR THE SURVEYING, SAMPLING AND LABORATORY ANALYSIS OF PHYTOBENTHOS IN SHALLOW RUNNING WATER
BS EN ISO 5667-3:2012 Water quality. Sampling Preservation and handling of water samples
DIN EN 16695 E : 2015 WATER QUALITY - GUIDANCE ON THE ESTIMATION OF PHYTOPLANKTON BIOVOLUME
UNE-EN ISO 5667-3:2013 Water quality - Sampling - Part 3: Preservation and handling of water samples (ISO 5667-3:2012)
13/30291479 DC : 0 BS EN 16695 - WATER QUALITY - GUIDANCE ON THE ESTIMATION OF MICROALGAL BIOVOLUME
DIN EN 15708:2010-03 Water quality - Guidance standard for the surveying, sampling and laboratory analysis of phytobenthos in shallow running water
07/30167044 DC : DRAFT AUG 2007 BS EN 15708 - WATER QUALITY - GUIDANCE STANDARD FOR THE SURVEYING, SAMPLING AND LABORATORY ANALYSIS OF PHYTOBENTHOS IN SHALLOW RUNNING WATER
I.S. EN 15972:2011 WATER QUALITY - GUIDANCE ON QUANTITATIVE AND QUALITATIVE INVESTIGATIONS OF MARINE PHYTOPLANKTON
I.S. EN 16695:2015 WATER QUALITY - GUIDANCE ON THE ESTIMATION OF PHYTOPLANKTON BIOVOLUME
DIN EN ISO 5667-3:2013-03 WATER QUALITY - SAMPLING - PART 3: PRESERVATION AND HANDLING OF WATER SAMPLES (ISO 5667-3:2012)
I.S. EN 16698:2015 WATER QUALITY - GUIDANCE ON QUANTITATIVE AND QUALITATIVE SAMPLING OF PHYTOPLANKTON FROM INLAND WATERS
I.S. EN ISO 5667-3:2012 WATER QUALITY - SAMPLING - PART 3: PRESERVATION AND HANDLING OF WATER SAMPLES (ISO 5667-3:2012)
I.S. EN 15708:2009 WATER QUALITY - GUIDANCE STANDARD FOR THE SURVEYING, SAMPLING AND LABORATORY ANALYSIS OF PHYTOBENTHOS IN SHALLOW RUNNING WATER
UNE-EN 16695:2016 Water quality - Guidance on the estimation of phytoplankton biovolume
EN 15708:2009 Water quality - Guidance standard for the surveying, sampling and laboratory analysis of phytobenthos in shallow running water
EN 15972:2011 Water quality - Guidance on quantitative and qualitative investigations of marine phytoplankton
EN 16698:2015 Water quality - Guidance on quantitative and qualitative sampling of phytoplankton from inland waters
EN 16695:2015 Water quality - Guidance on the estimation of phytoplankton biovolume
BS EN 15972:2011 Water quality. Guidance on quantitative and qualitative investigations of marine phytoplankton
DIN EN 15972:2011-11 Water quality - Guidance on quantitative and qualitative investigations of marine phytoplankton
UNI EN 15972 : 2012 WATER QUALITY - GUIDANCE ON QUANTITATIVE AND QUALITATIVE INVESTIGATIONS OF MARINE PHYTOPLANKTON
ISO 5667-3:2012 Water quality Sampling Part 3: Preservation and handling of water samples

ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories
EN 14407:2014 Water quality - Guidance for the identification and enumeration of benthic diatom samples from rivers and lakes
EN 14996:2006 Water quality - Guidance on assuring the quality of biological and ecological assessments in the aquatic environment
EN 13946:2014 Water quality - Guidance for the routine sampling and preparation of benthic diatoms from rivers and lakes

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