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EN ISO 13366-2:2006/AC:2007

Current
Current

The latest, up-to-date edition.

MILK - ENUMERATION OF SOMATIC CELLS - PART 2: GUIDANCE ON THE OPERATION OF FLUORO-OPTO-ELECTRONIC COUNTERS
Published date

13-06-2007

Foreword
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Factors affecting the results of measurements
   5.1 Sample bottles
   5.2 Sampling
   5.3 Preservation
   5.4 Sample storage and transport
   5.5 Interfering substances
   5.6 Sample quality at analysis
   5.7 Applied chemicals
   5.8 Instrument condition
   5.9 Working factor
   5.10 Testing volumes
6 Calibration
   6.1 Reference materials
   6.2 Calibration procedure
7 Sampling
8 Determination
9 Checks on performance in routine operation
   9.1 Blank checks
   9.2 Carry-over effect
   9.3 Ratio of reagent volume and test sample volume
   9.4 Pilot checks
   9.5 Additional instrument surveillance
   9.6 Repeatability
   9.7 Intralaboratory reproducibility
   9.8 External comparisons
10 Specific remarks for the use with milk from different
   species
   10.1 General
   10.2 Cow milk
   10.3 Goat milk
   10.4 Sheep milk
   10.5 Buffalo milk
11 Precision
   11.1 Repeatability
   11.2 Intralaboratory reproducibility
   11.3 Reproducibility
12 Test report
Bibliography

Provides guidance on the operating conditions for counting somatic cells, in both raw and chemically preserved milk, using fluoro-opto-electronic somatic cell counters in which either a rotating disc technique or flow cytometry is applied in the counting section.

Committee
TC 302
DevelopmentNote
Supersedes EN ISO 13366-3. (07/2007)
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current
Supersedes

ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 13366-1:2008 Milk Enumeration of somatic cells Part 1: Microscopic method (Reference method)
ISO 707:2008 Milk and milk products — Guidance on sampling
ISO/IEC Guide 43-1:1997 Proficiency testing by interlaboratory comparisons Part 1: Development and operation of proficiency testing schemes
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 8196-2:2009 Milk — Definition and evaluation of the overall accuracy of alternative methods of milk analysis — Part 2: Calibration and quality control in the dairy laboratory
ISO 8196-1:2009 Milk — Definition and evaluation of the overall accuracy of alternative methods of milk analysis — Part 1: Analytical attributes of alternative methods
ISO Guide 34:2009 General requirements for the competence of reference material producers

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