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EN ISO 20541:2008

Current
Current

The latest, up-to-date edition.

Milk and milk products - Determination of nitrate content - Method by enzymatic reduction and molecular-absorption spectrometry after Griess reaction (ISO 20541:2008)
Published date

01-09-2008

Foreword
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Preparation of test sample
   8.1 Dried milk, dried whey and milk
        protein concentrates
   8.2 Caseins and caseinates
   8.3 Cheese
   8.4 Whey cheese
9 Procedure
   9.1 Preparation of the test portion
   9.2 Removal of fat and protein
   9.3 Reagent blank test
   9.4 Determination
   9.5 Calibration
10 Calculation and expression of results
   10.1 Nitrite (matrix blank) content
   10.2 Total nitrite/nitrate content
   10.3 Nitrate content
11 Precision
   11.1 Interlaboratory testing
   11.2 Repeatability
   11.3 Reproducibility
12 Test report
Annex A (informative) - Interlaboratory trials
Bibliography

ISO 20541|IDF 197:2008 specifies a method for the determination of the nitrate content of milk and milk products by molecular-absorption spectrometry after Griess reaction (preceded by enzymatic reduction).The method is, in particular, applicable to whole, partly skimmed, skimmed and dried milk, hard, semi-hard and soft cheeses, processed cheese, whey cheese, caseins, caseinates, dried whey and milk protein concentrates.The method can be used at contents corresponding to a measured concentration in the sample solution (with blank subtracted) of more than 0,2 mg/l.

Committee
CEN/TC 302
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

ISO 707:2008 Milk and milk products — Guidance on sampling
ISO 1042:1998 Laboratory glassware One-mark volumetric flasks
ISO 648:2008 Laboratory glassware — Single-volume pipettes
ISO 835:2007 Laboratory glassware — Graduated pipettes
ISO 565:1990 Test sieves — Metal wire cloth, perforated metal plate and electroformed sheet — Nominal sizes of openings
EN 12014-3:2005 Foodstuffs - Determination of nitrate and/or nitrite content - Part 3: Spectrometric determination of nitrate and nitrite content of meat products after enzymatic reduction of nitrate to nitrite
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods

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