Name: EN ISO 22160:2007
Brand: CEN

Milk and milk-based drinks - Determination of alkaline phosphatase activity - Enzymatic photo-activated system (EPAS) method (ISO 22160:2007)

Published date

01-04-2007

Publisher

Comite Europeen de Normalisation

Foreword
1 Scope
2 Terms and definitions
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparations
   7.1 Test samples
   7.2 Alkaline phosphatase-free milk
8 Procedure
   8.1 Calibration
   8.2 Calibration adjustment
   8.3 Control tests and calibration check
   8.4 Determination
   8.5 Heat-resistant microbial alkaline phosphatase
        control
9 Calculation and expression of results
   9.1 General
   9.2 Manual calculation
   9.3 Expression of results
10 Precision
   10.1 Interlaboratory test
   10.2 Repeatability
   10.3 Reproducibility
11 Test report
Annex A (informative) Procedure flowchart
Annex B (informative) Results of interlaboratory
        trials
Bibliography

ISO 22160|IDF 209:2007 specifies a method for the determination of the alkaline phosphatase activity in pasteurized whole milk, semi-skimmed milk, skimmed milk, cream and flavoured milks using a chemiluminescent (EPAS) method.The method is applicable to milk and milk-based drinks from cows, sheep, buffalo and goats. The method is also suitable for any liquid-based sample if diluted in such a way that the diluted alkaline phosphatase activity has less than 7 000 milliunits per litre.

Committee	CEN/TC 302
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
NEN EN ISO 22160 : 2007	Identical
PN EN ISO 22160 : 2009	Identical
UNI EN ISO 22160 : 2007	Identical
ISO 22160:2007	Identical
I.S. EN ISO 22160:2007	Identical
NS EN ISO 22160 : 1ED 2007	Identical
NBN EN ISO 22160 : 2007	Identical
NF EN ISO 22160 : 2007	Identical
SN EN ISO 22160 : 2007	Identical
UNE-EN ISO 22160:2007	Identical
DIN EN ISO 22160:2007-08	Identical
BS EN ISO 22160:2007	Identical

ISO 5725-1:1994	Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 11816-1:2013	Milk and milk products — Determination of alkaline phosphatase activity — Part 1: Fluorimetric method for milk and milk-based drinks
ISO 11816-2:2016	Milk and milk products — Determination of alkaline phosphatase activity — Part 2: Fluorimetric method for cheese
ISO 707:2008	Milk and milk products — Guidance on sampling
ISO 5725-2:1994	Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method