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I.S. EN ISO 15216-1:2017

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS USING REAL-TIME RT-PCR - PART 1: METHOD FOR QUANTIFICATION (ISO 15216-1:2017)
Available format(s)

Hardcopy , PDF

Superseded date

31-05-2021

Language(s)

English

Published date

01-01-2017

Preview

For Harmonized Standards, check the EU site to confirm that the Standard is cited in the Official Journal.

Only cited Standards give presumption of conformance to New Approach Directives/Regulations.

National Foreword
European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Equipment and consumables
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Precision
12 Test report
Annex A (normative) - Diagram of procedure
Annex B (normative) - Composition and preparation of reagents
        and buffers
Annex C (informative) - Real-time RT-PCR mastermixes and
        cycling parameters
Annex D (informative) - Real-time RT-PCR primers and
        hydrolysis probes for the detection of HAV, norovirus
        GI and GII and mengo virus (process control)
Annex E (informative) - Growth of mengo virus strain MC[0]
        for use as a process control
Annex F (informative) - RNA extraction using the NucliSENS[R]
        system
Annex G (informative) - Generation of dsDNA control stocks
Annex H (informative) - Generation of EC RNA stocks
Annex I (informative) - Typical optical plate layout
Annex J (informative) - Method validation studies and
        performance characteristics
Bibliography

Describes a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces.

DevelopmentNote
Supersedes SR CEN ISO/TS 15216-1. (05/2017)
DocumentType
Standard
Pages
66
PublisherName
National Standards Authority of Ireland
Status
Superseded
SupersededBy
Supersedes

Standards Relationship
EN ISO 15216-1:2017 Identical
ISO 15216-1:2017 Identical

ISO 22174:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 20838:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 17468:2016 Microbiology of the food chain — Technical requirements and guidance on establishment or revision of a standardized reference method
ISO 22119:2011 Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions

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