MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF POTENTIALLY ENTEROPATHOGENIC VIBRIO SPP. - PART 1: DETECTION OF VIBRIO PARAHAEMOLYTICUS AND VIBRIO CHOLERAE (ISO 21872-1:2017)

Amended by

I.S. EN ISO 21872-1:2017/A1:2023

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

01-01-2017

Publisher

National Standards Authority of Ireland

Preview

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National Foreword
European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Culture media and reagents
6 Equipment and consumables
7 Sampling
8 Preparation of the test sample
9 Procedure
10 Expression of results
11 Performance characteristics of the method
12 Test report
Annex A (normative) - Diagram of procedure
Annex B (normative) - Composition and preparation
        of the culture media and reagents
Annex C (informative) - Conventional PCR for the
        detection of Vibrio parahaemolyticus,
        thermostable direct haemolysin (tdh) and
        thermostable direct related haemolysin (trh)
        genes, Vibrio cholerae and Vibrio vulnificus
Annex D (informative) - Real-time PCR for the
        detection of Vibrio parahaemolyticus,
        thermostable direct haemolysin gene (tdh)
        and Vibrio vulnificus
Annex E (informative) - Results of an interlaboratory
        study
Bibliography

Defines a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract.

DocumentType	Standard
Pages	50
PublisherName	National Standards Authority of Ireland
Status	Current

Standards	Relationship
ISO 21872-1:2017	Identical
EN ISO 21872-1:2017	Identical

ISO 22174:2005	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 5725-1:1994	Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 6887-1:2017	Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO/TS 20836:2005	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Performance testing for thermal cyclers
ISO 20837:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for sample preparation for qualitative detection
ISO 22118:2011	Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics
ISO 20838:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 11133:2014	Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
ISO 5725-2:1994	Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 6887-3:2017	Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products
ISO 17468:2016	Microbiology of the food chain — Technical requirements and guidance on establishment or revision of a standardized reference method
ISO 22119:2011	Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions

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I.S. EN ISO 21872-1:2017

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