MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

01-01-2005

Publisher

National Standards Authority of Ireland

Preview

For Harmonized Standards, check the EU site to confirm that the Standard is cited in the Official Journal.

Only cited Standards give presumption of conformance to New Approach Directives/Regulations.

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
   4.1 General
   4.2 Preliminary microbial enrichment
   4.3 Nucleic acid preparation
   4.4 PCR amplification
   4.5 Detection and confirmation of PCR products
5 Test material
6 General laboratory requirements
   6.1 General
   6.2 Personnel
   6.3 Laboratory setup
   6.4 Waste management
7 Reagents
8 Apparatus and equipment
   8.1 General
   8.2 Special considerations
9 Procedure
   9.1 Sample preparation
   9.2 Amplification
   9.3 Control reaction
   9.4 Confirmation of PCR results
10 Evaluation
11 Test report
Bibliography

Describes the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA).

DocumentType	Standard
Pages	22
PublisherName	National Standards Authority of Ireland
Status	Current

Standards	Relationship
ISO 22174:2005	Identical
BS EN ISO 22174:2005	Identical
UNE-EN ISO 22174:2005	Identical
UNI EN ISO 22174 : 2005	Identical
EN ISO 22174:2005	Identical
DIN EN ISO 22174:2005-05	Identical