ISO 11063:2012
Withdrawn
A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.
A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.
Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users
22-10-2020
French, English
06-02-2012
This International Standard specifies a method for direct extraction of DNA from soil samples to analyse the
global structure and the abundance of soil bacterial communities using PCR-based technologies. This method
is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in
organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.
The direct extraction of DNA from soil samples provides unique insight into the richness and structure of
microbial communities which are key parameters to estimate the biodiversity of soil microbiota. Molecular
approaches based on PCR (polymerase chain reaction) amplification of soil DNA constitute a promising
domain and can contribute in the near future to the development of routine tools to monitor the microbiota of
soil environments.
Users of the method ought to be aware that although soil submitted to the DNA extraction procedure is sieved
thoroughly (2 mm mesh, procedure described in 5.1), plant residues can still remain in soil samples and, as a
result, traces of plant DNA can contaminate the soil DNA extract.
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