Soil quality — Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

French, English

Published date

12-01-2016

Publisher

International Organization for Standardization

ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.

It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.

Committee	ISO/TC 190/SC 4
DevelopmentNote	Supersedes ISO/DIS 17601. (01/2016)
DocumentType	Standard
Pages	32
PublisherName	International Organization for Standardization
Status	Current

Standards	Relationship
SS-EN ISO 17601 : 2018	Identical
EN ISO 17601:2018	Identical
UNI EN ISO 17601 : 2018	Identical
SN EN ISO 17601:2018	Identical
UNE-EN ISO 17601:2018	Identical
I.S. EN ISO 17601:2018	Identical
NEN EN ISO 17601 : 2018	Identical
NF EN ISO 17601 : 2018	Identical
NS EN ISO 17601 : 2018	Identical
BS EN ISO 17601:2018	Identical
NF ISO 17601 : 2016	Identical

DIN ISO 19204 E : 2017	SOIL QUALITY - PROCEDURE FOR SITE-SPECIFIC ECOLOGICAL RISK ASSESSMENT OF SOIL CONTAMINATION (SOIL QUALITY TRIAD APPROACH) (ISO 19204:2017)
17/30352199 DC : 0	BS ISO 15799 - SOIL QUALITY - GUIDANCE ON THE ECOTOXICOLOGICAL CHARACTERIZATION OF SOILS AND SOIL MATERIALS
ISO 19204:2017	Soil quality — Procedure for site-specific ecological risk assessment of soil contamination (soil quality TRIAD approach)
BS ISO 19204:2017	Soil quality. Procedure for site-specific ecological risk assessment of soil contamination (soil quality TRIAD approach)
DIN ISO 19204:2015-09 (Draft)	SOIL QUALITY - PROCEDURE FOR SITE-SPECIFIC ECOLOGICAL RISK ASSESSMENT OF SOIL CONTAMINATION (SOIL QUALITY TRIAD APPROACH) (ISO 19204:2017)

ISO 10381-6:2009	Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 11063:2012	Soil quality Method to directly extract DNA from soil samples

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ISO 17601:2016

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