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NEN EN ISO 14939 : 2001

Current
Current

The latest, up-to-date edition.

ANIMAL FEEDING STUFFS - DETERMINATION OF CARBADOX CONTENT - METHOD USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
Published date

12-01-2013

1 Scope
2 Normative reference
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparation of test sample
8 Procedure
   8.1 General
   8.2 Preparation of spiked sample
   8.3 Extraction
   8.4 Column chromatography
   8.5 HPLC analysis
9 Confirmation
   9.1 General
   9.2 Co-chromatography
   9.3 Diode array detector
   9.4 Post-column derivatization
10 Calculation of results
   10.1 General
   10.2 Feeding stuffs containing 0,1 mg/kg to 10 mg/kg
        of carbadox
   10.3 Feeding stuffs containing 0,1 mg/kg to 100 mg/kg
        of carbadox
   10.4 Premixtures containing up to 10 % of carbadox
11 Precision
   11.1 Interlaboratory test
   11.2 Repeatability
   11.3 Reproducibility
12 Test report
Annex A (informative) Flow chart
Annex B (informative) Results of interlaboratory test
Bibliography

Describes a high-performance liquid chromatographic (HPLC) method for the determination of the carbadox content in premixtures and animal feeding stuffs. This method is applicable to animal feeding stuffs with a mass fraction of carbadox of 0,5 mg/kg (limit of quantification) to 100 mg/kg, and to premixtures with a mass fraction of carbadox up to 10 %. The lower limit of detection is 0,1 mg/kg.

DocumentType
Standard
PublisherName
Netherlands Standards
Status
Current

Standards Relationship
DIN EN ISO 14939:2001-12 Identical
EN ISO 14939:2001 Identical
ISO 14939:2001 Identical
UNI EN ISO 14939 : 2002 Identical
UNE-EN ISO 14939:2002 Identical
BS EN ISO 14939:2001 Identical

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