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NS EN ISO 22174 : 1ED 2005

Current
Current

The latest, up-to-date edition.

MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
Published date

12-01-2013

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
   4.1 General
   4.2 Preliminary microbial enrichment
   4.3 Nucleic acid preparation
   4.4 PCR amplification
   4.5 Detection and confirmation of PCR products
5 Test material
6 General laboratory requirements
   6.1 General
   6.2 Personnel
   6.3 Laboratory setup
   6.4 Waste management
7 Reagents
8 Apparatus and equipment
   8.1 General
   8.2 Special considerations
9 Procedure
   9.1 Sample preparation
   9.2 Amplification
   9.3 Control reaction
   9.4 Confirmation of PCR results
10 Evaluation
11 Test report
Bibliography

Describes the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). Applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne pathogens using the polymerase chain reaction (PCR).

DocumentType
Standard
PublisherName
Norwegian Standards (Norges Standardiseringsforbund)
Status
Current

Standards Relationship
ISO 22174:2005 Identical
BS EN ISO 22174:2005 Identical
UNE-EN ISO 22174:2005 Identical
UNI EN ISO 22174 : 2005 Identical
EN ISO 22174:2005 Identical
DIN EN ISO 22174:2005-05 Identical

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