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S.R. CEN ISO/TS 15216-2:2013

Withdrawn
Withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

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withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

MICROBIOLOGY OF FOOD AND ANIMAL FEED - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS IN FOOD USING REAL-TIME RT-PCR - PART 2: METHOD FOR QUALITATIVE DETECTION (ISO/TS 15216-2:2013, CORRECTED VERSION 2013-05-01)
Available format(s)

Hardcopy , PDF

Withdrawn date

31-03-2020

Language(s)

English

Published date

01-01-2013

Preview

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus and materials
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Test report
Annex A (normative) - Diagram of procedure
Annex B (informative) - Real-time RT-PCR mastermixes
        and cycling parameters
Annex C (informative) - Real-time RT-PCR primers and
        hydrolysis probes for the detection of HAV, norovirus
        GI and GII and mengo virus (process control)
Annex D (informative) - Growth of mengo virus strain MC[0]
        for use as a process control
Annex E (informative) - RNA extraction using the BioMerieux
        NucliSens[R] system
Annex F (normative) - Composition and preparation of reagents
        and buffers
Annex G (informative) - Generation of external control RNA
        (EC RNA) stocks
Annex H (informative) - Typical optical plate layout
Bibliography

Defines a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces.

DocumentType
Standard
Pages
43
PublisherName
National Standards Authority of Ireland
Status
Withdrawn
SupersededBy

Standards Relationship
ISO/TS 15216-2:2013 Identical
CEN ISO/TS 15216-2:2013 Identical

ISO 22174:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 22118:2011 Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics
ISO 22119:2011 Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions

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