Monitoring the effects of genetically modified organisms (GMOs) - Method for extracting nucleic acids from soil for the analysis of microbial communities and detection of transgenic DNA - Quality requirements and applications

Available format(s)

Hardcopy , PDF

Language(s)

German - English

Published date

01-08-2013

Publisher

Verlag des Vereins Deutscher Ingenieure

Preliminary note
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Quality requirements for nucleic
  acid extracts
5 List of materials
6 Procedure
Annex A - Protocol for DNA extraction
          from soil samples
Annex B - Special modification of a
          Protocol for DNA extraction
          from soils with a higher amount
          of humic material
Annex C - Protocol for simultaneous
          extraction of DNA and RNA
          from soil samples
Bibliography

Nucleic acids extracted from soil can serve as a starting material for the detection of microorganisms living in soil and for assessing their diversity.

DevelopmentNote	Supersedes VDI 4331-7. (08/2013)
DocumentType	Standard
Pages	27
PublisherName	Verlag des Vereins Deutscher Ingenieure
Status	Current
Supersedes	VDI 4331 Blatt 7:2011-11 (Draft)

VDI 4331 Blatt 1:2014-01	Monitoring the effects of genetically modified organisms - Effects on soil organisms
VDI 4330 Blatt 5:2015-11	Monitoring the effects of genetically modified organisms (GMOs) - Guidelines for the collection and preparation of plant samples for molecular biological analysis

VDI 4331 Blatt 1:2014-01	Monitoring the effects of genetically modified organisms - Effects on soil organisms
EN ISO 21571:2005/A1:2013	FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - NUCLEIC ACID EXTRACTION (ISO 21571:2005/AMD 1:2013)
ISO 10381-1:2002	Soil quality Sampling Part 1: Guidance on the design of sampling programmes
ISO 10381-6:2009	Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 24276:2006	Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products General requirements and definitions
VDI 4330 Blatt 1:2006-10	Monitoring the ecological effects of genetically modified organisms - Genetically modified plants - Basic principles and strategies
ISO 21571:2005	Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Nucleic acid extraction
VDI 1000:2017-02	VDI Standardisation Work - Principles and procedures
DIN EN ISO 21571:2013-08	FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - NUCLEIC ACID EXTRACTION (ISO 21571:2005 + AMD 1:2013)
VDI 4330 Blatt 7:2006-12	Monitoring the effects of genetically modified organisms (GMO) - Qualitative methods for the detection of genetically engineered nucleic acids in the environment
EN ISO 24276:2006/A1:2013	FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - GENERAL REQUIREMENTS AND DEFINITIONS (ISO 24276:2006/AMD 1:2013)

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VDI 4331 Blatt 2:2013-08

Monitoring the effects of genetically modified organisms (GMOs) - Method for extracting nucleic acids from soil for the analysis of microbial communities and detection of transgenic DNA - Quality requirements and applications

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Abstract

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Standards Referencing This Book

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