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AS 5132:2017

Current
Current

The latest, up-to-date edition.

Waters - Examination for Legionella spp. including Legionella pneumophila - Using concentration
Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

English

Published date

10-05-2017

Preview

Specifies a method for isolating and estimating the number of Legionella pneumophila and a range of other Legionella spp. in water with low microbial loads.

This Standard sets out a method for isolating and estimating the number of Legionella pneumophila and a range of other Legionella spp. in water with low microbial loads. The target matrix for this Standard is water samples from reticulated water systems where colonisation by Legionella spp. is of public health concern. While this Standard is not restricted to this matrix, it may not be suitable for some environmental and recreational samples, such as industrial, waste and natural waters. This is because samples are concentrated and the high microbial loads in these samples could interfere with the detection of Legionella spp., leading to the possibility of false negative results.This Standard has a lower limit of detection than AS 3896. Therefore, it is suitable when—(a) the detection of lower levels is sought; or(b) higher precision is desired for levels close to the lower limit of detection of AS 3896.NOTES: 1 A flow chart of the procedure is shown in Appendix A.2 This method will isolate L. pneumophila but not all other Legionella spp. L. pneumophila serogroup 1 accounts for the majority of legionellae infections.3 Conditions that favour the isolation of L. pneumophila do not necessarily apply to some Legionella spp. However, treatments and media included in this method are designed to enhance the recovery of other species in addition to L. pneumophila.

Committee
FT-020
DocumentType
Standard
ISBN
978-1-76035-746-7
Pages
23
PublisherName
Standards Australia
Status
Current
Supersedes

First published as AS 5132:2017.

AS/NZS 2243.3:2010 Safety in laboratories Microbiological safety and containment
AS/NZS 4276.1:2007 Water microbiology General information and procedures (ISO 8199:2005, MOD)
AS 3896:2017 Waters - Examination for Legionella spp. Including Legionella pneumophila
AS 2031-2012 Water quality - Sampling for microbiological analysis (ISO 19458:2006, MOD)

AS 3896:2017 Waters - Examination for Legionella spp. Including Legionella pneumophila

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