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  • ASTM D 4012 : 2015

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    Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water

    Available format(s):  Hardcopy, PDF

    Language(s):  English

    Published date:  01-07-2015

    Publisher:  American Society for Testing and Materials

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    1.1This test method covers a protocol for capturing, extracting and quantifying the cellular adenosine triphosphate (cATP) content associated with microorganisms normally found in laboratory cultures, waters, wastewaters, and in plankton and periphyton samples from waters.

    1.2The ATP is measured using a bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the samples. The light is produced and measured quantitatively as relative light units (RLU) which are converted by comparison with an ATP standard and computation to pg ATP/mL.

    1.3This method does not remove all known chemical interferences, known to either luminesce in the 530 ± 20 nm range, or to quench light emitted in that range. It should not be used to determine ATP concentrations in samples with dissolved organic compounds, heavy metals or >10 000 ppm total dissolved solids. Alternative methods have been developed for determining ATP concentrations in fluids samples likely to contain such interferences (Test Methods D7687 and E2694).

    1.4Knowledge of the concentration of ATP can be related to viable biomass or metabolic activity of microorganisms (Appendix X1).

    1.5This test method offers a high degree of sensitivity, rapidity, accuracy, and reproducibility.

    1.6The analyst should be aware that the precision statement pertains only to determinations in reagent water and not necessarily in the matrix being tested.

    1.7This test method is equally suitable for use in the laboratory or field.

    1.8The method normally detects cATP concentrations in the range of 0.1 pg cATP/mL (–1.0Log10 [pg cATP/mL]) to 4 000 000 pg cATP/mL (6.6 Log10 [pg cATP/mL]) in 50 mL water samples.

    1.9Providing interferences can be overcome, bioluminescence is a reliable and proven method for qualifying and quantifying ATP, although the method does not differentiate between ATP from different sources, for example, from different types of microorganisms, such as bacteria, fungi, algae and protozoa.

    1.10The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.11This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

    General Product Information - (Show below) - (Hide below)

    Committee D 19
    Document Type Test Method
    Publisher American Society for Testing and Materials
    Status Current
    Supersedes

    Standards Referenced By This Book - (Show below) - (Hide below)

    ASTM D 6469 : 2017 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
    ASTM E 645 : 2018 Standard Practice for Evaluation of Microbicides Used in Cooling Water Systems
    ASTM E 2694 : 2016 Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
    ASTM D 7687 : 2017 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
    ASTM D 7847 : 2017 Standard Guide for Interlaboratory Studies for Microbiological Test Methods
    ASTM D 7463 : 2018 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
    ASTM E 1326 : 2015 : REV A Standard Guide for Evaluating Non-culture Microbiological Tests

    Standards Referencing This Book - (Show below) - (Hide below)

    ASTM D 4175 : 2020 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2014 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2009 : REV A : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 6300 : 2020 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2008 : REV B : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 6300 : 2013 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4175 : 2005 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 6300 : 2014 : REV A : EDT 1 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM F 1671 : 1997 : REV B Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Blood-Borne Pathogens Using Phi-X174 Bacteriophage Penetration as a Test System
    ASTM D 4175 : 2005 : EDT 2 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 1129 : 2003 Standard Terminology Relating to Water
    ASTM D 6161 : 1998 Standard Terminology Used for Crossflow Microfiltration, Ultrafiltration, Nanofiltration, and Reverse Osmosis Membrane Processes
    ASTM D 4175 : 2015 : REV C Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2020 : REV B Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2017 : REV A : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2017 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6161 : 2019 Standard Terminology Used for Microfiltration, Ultrafiltration, Nanofiltration, and Reverse Osmosis Membrane Processes
    ASTM D 1129 : 2013 Standard Terminology Relating to Water
    ASTM D 7687 : 2017 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
    ASTM D 1129 : 2013 : R2020 Standard Terminology Relating to Water
    ASTM D 5847 : 1999 : REV A : EDT 1 Standard Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
    ASTM D 6300 : 2017 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4175 : 2008 : REV A Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2020 : REV A Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2017 : REV B Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2009 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 1193 : 1977 : R1983 : EDT 1 Standard Specification for Reagent Water
    ASTM D 5847 : 2002 : R2007 Standard Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
    ASTM D 1601 : 1999 Standard Test Method for Dilute Solution Viscosity of Ethylene Polymers
    ASTM D 6300 : 2016 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4175 : 2009 : REV A : EDT 4 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2009 : REV A : EDT 3 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 6300 : 2004 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM E 2694 : 2011 Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
    ASTM E 2694 : 2009 Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
    ASTM D 1129 : 2006 Standard Terminology Relating to Water
    ASTM D 4175 : 2018 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2019 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4175 : 2001 : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2020 : REV B : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2001 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2005 : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 5847 : 2002 : R2012 Standard Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
    ASTM D 6300 : 2021 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2019 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4175 : 2002 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 6300 : 2020 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 7687 : 2011 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel, Fuel/Water Mixtures, and Fuel-Associated Water with Sample Concentration by Filtration
    ASTM D 1129 : 1999 : REV A Standard Terminology Relating to Water
    ASTM D 1129 : 2006 : REV A Standard Terminology Relating to Water
    ASTM D 4175 : 2015 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 1193 : 2006 : R2011 Standard Specification for Reagent Water
    ASTM D 4175 : 2002 : REV A : EDT 2 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 5847 : 2002 : R2020 Standard Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
    ASTM D 7687 : 2021 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
    ASTM D 1129 : 2003 : REV A Standard Terminology Relating to Water
    ASTM D 1129 : 2002 Standard Terminology Relating to Water
    ASTM D 6300 : 2020 : REV B Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2006 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 1601 : 2018 Standard Test Method for Dilute Solution Viscosity of Ethylene Polymers
    ASTM D 4175 : 2009 : REV A : EDT 5 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2016 : REV C Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2015 : REV B Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 6300 : 2014 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6300 : 2015 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4175 : 2015 : REV A Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 6300 : 1999 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 1193 : 1991 Standard Specification for Reagent Water
    ASTM D 4175 : 2009 : REV A Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2016 : REV B Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM F 1671 : 2007 Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Blood-Borne Pathogens Using Phi-X174 Bacteriophage Penetration as a Test System
    ASTM D 4175 : 2002 : REV A : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2008 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM E 2694 : 2016 Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
    ASTM D 4175 : 2002 : REV A Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 1129 : 2004 Standard Terminology Relating to Water
    ASTM D 1601 : 1978 Standard Test Method for Dilute Solution Viscosity Of Ethylene Polymers
    ASTM F 1671 : 2003 Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Blood-Borne Pathogens Using Phi-X174 Bacteriophage Penetration as a Test System
    ASTM D 4175 : 2000 : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 5847 : 2022 Standard Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
    ASTM D 4175 : 2016 : REV A Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2013 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6161 : 2005 Standard Terminology Used for Microfiltration, Ultrafiltration, Nanofiltration and Reverse Osmosis Membrane Processes
    ASTM D 4175 : 2019 : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2008 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6300 : 2003 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 1129 : 2002 : REV A Standard Terminology Relating to Water
    ASTM D 6300 : 2007 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 5847 : 2002 Standard Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
    ASTM D 4175 : 2017 : REV A Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2002 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM E 2694 : 2021 Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
    ASTM D 4175 : 2009 : REV A : EDT 2 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 1193 : 1970 Standard Specification For Reagent Water
    ASTM D 1601 : 2012 Standard Test Method for Dilute Solution Viscosity of Ethylene Polymers
    ASTM D 1193 : 2006 Standard Specification for Reagent Water
    ASTM D 4175 : 2018 : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2016 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2016 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 1193 : 1999 Standard Specification for Reagent Water
    ASTM D 1129 : 2010 Standard Terminology Relating to Water
    ASTM D 6161 : 2010 Standard Terminology Used for Microfiltration, Ultrafiltration, Nanofiltration and Reverse Osmosis Membrane Processes (Withdrawn 2019)
    ASTM D 1129 : 2006 : REV A : EDT 1 Standard Terminology Relating to Water
    ASTM D 1129 : 2001 Standard Terminology Relating to Water
    ASTM D 1129 : 2004 : EDT 1 Standard Terminology Relating to Water
    ASTM D 4175 : 2017 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2019 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 1193 : 2006 : R2018 Standard Specification for Reagent Water
    ASTM D 6300 : 2014 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4175 : 2015 : REV B : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2008 : REV B Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
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