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ASTM D 4454 : 1985 : R2009

ASTM D 4454 : 1985 : R2009

Withdrawn

Withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy (Withdrawn 2015)

Available format(s)

Hardcopy , PDF

Withdrawn date

31-12-2015

Language(s)

English

Published date

01-05-2009

Publisher

American Society for Testing and Materials

CONTAINED IN VOL. 11.02, 2015 Defines the detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure.

1.1 This test method covers the detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. This test method is applicable to environmental waters and potable waters.

1.2 Certain types of debris and other microorganisms may fluoresce in acridine-orange stained smears.

1.3 The procedure described requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.

1.4 Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.

1.5 Approximately 104 cells/mL are required for detection by this test method.

1.6 Minimal cell size which allows the detection of formazan deposits is represented by bacteria of 0.4 μm.2

1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.8 This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Committee
D 19
DocumentType
Test Method
Pages
3
ProductNote
Reconfirmed 2009
PublisherName
American Society for Testing and Materials
Status
Withdrawn

ASTM E 979 : 2009 : R2015 Standard Practice for Evaluation of Antimicrobial Agents as Preservatives for Invert Emulsion and Other Water Containing Hydraulic Fluids
ASTM D 6469 : 2017 Standard Guide for Microbial Contamination in Fuels and Fuel Systems

ASTM D 1129 : 2013 : REDLINE Standard Terminology Relating to Water

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PDF - English
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