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ASTM D 4455 : 1985 : R2014

Withdrawn
Withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure (Withdrawn 2019)
Available format(s)

Hardcopy , PDF

Withdrawn date

02-04-2019

Language(s)

English

Published date

01-01-2014

Publisher

American Society for Testing and Materials

CONTAINED IN VOL. 11.02, 2015 Defines a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure.

1.1This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. It is applicable to environmental waters.

1.2Certain types of debris and other microorganisms may fluoresce in acridine orange-stained smears.

1.3The test method requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.2

1.4Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.

1.5Approximately 104 cells/mL are required for detection by this test method.2

1.6The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Committee
D 19
DocumentType
Test Method
Pages
3
ProductNote
Reconfirmed 2014
PublisherName
American Society for Testing and Materials
Status
Withdrawn
Supersedes

ASTM E 2800 : 2011 : R2017 Standard Practice for Characterization of <emph type="ital">Bacillus</emph> Spore Suspensions for Reference Materials

ASTM D 1129 : 2013 : REDLINE Standard Terminology Relating to Water

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Hardcopy - English
PDF - English
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