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  • ASTM D 7847 : 2017

    Superseded A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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    Standard Guide for Interlaboratory Studies for Microbiological Test Methods

    Available format(s):  Hardcopy, PDF

    Superseded date:  23-08-2022

    Language(s):  English

    Published date:  28-06-2017

    Publisher:  American Society for Testing and Materials

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    Abstract - (Show below) - (Hide below)

    1.1 Microbiological test methods present challenges that are unique relative to chemical or physical parameters, because microbes proliferate, die off and continue to be metabolically active in samples after those samples have been drawn from their source.

    Scope - (Show below) - (Hide below)

    1.1Microbiological test methods present challenges that are unique relative to chemical or physical parameters, because microbes proliferate, die off and continue to be metabolically active in samples after those samples have been drawn from their source.

    1.1.1Microbial activity depends on the presence of available water. Consequently, the detection and quantification of microbial contamination in fuels and lubricants is made more complicated by the general absence of available water from these fluids.

    1.1.2Detectability depends on the physiological state and taxonomic profile of microbes in samples. These two parameters are affected by various factors that are discussed in this guide, and contribute to microbial data variability.

    1.2This guide addresses the unique considerations that must be accounted for in the design and execution of interlaboratory studies intended to determine the precision of microbiological test methods designed to quantify microbial contamination in fuels, lubricants and similar low water-content (water activity <0.8) fluids.

    1.3This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

    1.4This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

    General Product Information - (Show below) - (Hide below)

    Committee D 02
    Document Type Guide
    Publisher American Society for Testing and Materials
    Status Superseded
    Superseded By
    Supersedes

    Standards Referenced By This Book - (Show below) - (Hide below)

    ASTM D 8070 :2019 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
    ASTM D 7687 : 2017 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
    ASTM D 2777 : 2021 Standard Practice for Determination of Precision and Bias of Applicable Test Methods of Committee D19 on Water

    Standards Referencing This Book - (Show below) - (Hide below)

    ASTM D 4175 : 2020 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2014 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2009 : REV A : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM E 1326 : 1998 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
    ASTM D 6300 : 2020 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2008 : REV B : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 6469 : 2004 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
    ASTM D 6300 : 2013 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM E 1326 : 2015 Standard Guide for Evaluating Non-culture Microbiological Tests Used for Enumerating Bacteria
    ASTM D 6974 : 2016 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
    ASTM D 4175 : 2005 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4012 : 1981 : R2009 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
    ASTM D 7463 : 2016 : EDT 1 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
    ASTM D 6300 : 2014 : REV A : EDT 1 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 7463 : 2014 : REV A Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
    ASTM D 7464 : 2020 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
    ASTM D 4175 : 2005 : EDT 2 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4012 : 2015 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
    ASTM E 1326 : 2015 : REV A Standard Guide for Evaluating Non-culture Microbiological Tests
    ASTM D 1129 : 2003 Standard Terminology Relating to Water
    ASTM D 4175 : 2015 : REV C Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 6974 : 2009 : R2013 : EDT 2 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
    ASTM D 4175 : 2020 : REV B Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2017 : REV A : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2017 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 1129 : 2013 Standard Terminology Relating to Water
    ASTM D 7687 : 2017 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
    ASTM D 7463 : 2021 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
    ASTM D 1129 : 2013 : R2020 Standard Terminology Relating to Water
    ASTM D 6300 : 2017 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4175 : 2008 : REV A Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2020 : REV A Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4012 : 1981 : R2002 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
    ASTM D 4175 : 2017 : REV B Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2009 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM E 1326 : 2006 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
    ASTM D 6469 : 2012 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
    ASTM D 8070 : 2020 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
    ASTM D 6300 : 2016 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4175 : 2009 : REV A : EDT 4 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2009 : REV A : EDT 3 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 6469 : 2008 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
    ASTM D 6300 : 2004 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 1129 : 2006 Standard Terminology Relating to Water
    ASTM D 7463 : 2008 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated Water
    ASTM D 4175 : 2018 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6974 : 2009 : R2013 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
    ASTM D 7978 : 2014 Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water—Thixotropic Gel Culture Method
    ASTM D 6300 : 2019 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM E 1326 : 2008 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
    ASTM D 4175 : 2001 : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2020 : REV B : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2001 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2005 : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 7464 : 2019 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
    ASTM D 7978 : 2014 : R2019 Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water—Thixotropic Gel Culture Method
    ASTM D 7463 : 2015 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
    ASTM D 6300 : 2021 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2019 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4175 : 2002 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM E 1259 : 2010 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
    ASTM E 2756 : 2019 Standard Terminology Relating to Antimicrobial and Antiviral Agents
    ASTM D 6300 : 2020 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 7687 : 2011 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel, Fuel/Water Mixtures, and Fuel-Associated Water with Sample Concentration by Filtration
    ASTM D 8070 : 2019 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
    ASTM D 1129 : 1999 : REV A Standard Terminology Relating to Water
    ASTM D 7464 : 2008 : R2013 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
    ASTM D 1129 : 2006 : REV A Standard Terminology Relating to Water
    ASTM D 4175 : 2015 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM E 1601 : 2019 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
    ASTM D 4175 : 2002 : REV A : EDT 2 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 7687 : 2021 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
    ASTM D 6469 : 2014 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
    ASTM D 1129 : 2003 : REV A Standard Terminology Relating to Water
    ASTM D 1129 : 2002 Standard Terminology Relating to Water
    ASTM E 1601 : 1998 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
    ASTM D 6300 : 2020 : REV B Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2006 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM E 2756 : 2010 Standard Terminology Relating to Antimicrobial and Antiviral Agents
    ASTM E 1259 : 1994 Standard Test Method for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
    ASTM E 1601 : 2010 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
    ASTM D 7463 : 2018 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
    ASTM D 4175 : 2009 : REV A : EDT 5 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2016 : REV C Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2015 : REV B Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 8070 : 2021 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
    ASTM D 6300 : 2014 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6300 : 2015 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4175 : 2015 : REV A Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 6300 : 1999 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4175 : 2009 : REV A Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2016 : REV B Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2002 : REV A : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2008 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM E 1601 : 2012 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
    ASTM D 6974 : 2009 : R2013 : EDT 1 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
    ASTM D 4175 : 2002 : REV A Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 1129 : 2004 Standard Terminology Relating to Water
    ASTM D 6974 : 2009 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
    ASTM D 4012 : 1981 : R1997 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
    ASTM D 4175 : 2000 : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 4175 : 2016 : REV A Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2013 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 7464 : 2014 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
    ASTM D 4175 : 2019 : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 7463 : 2016 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
    ASTM D 6974 : 2003 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels-Filtration and Culture Procedures
    ASTM D 6974 : 2020 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
    ASTM E 1259 : 2005 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
    ASTM D 8070 : 2016 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
    ASTM D 6300 : 2008 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6300 : 2003 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6469 : 2017 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
    ASTM D 6469 : 2011 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
    ASTM D 1129 : 2002 : REV A Standard Terminology Relating to Water
    ASTM D 6300 : 2007 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM E 1259 : 2001 Standard Test Method for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
    ASTM E 2756 : 2018 Standard Terminology Relating to Antimicrobial and Antiviral Agents
    ASTM D 6469 : 1999 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
    ASTM D 4175 : 2017 : REV A Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2002 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4175 : 2009 : REV A : EDT 2 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM D 6469 : 2008 : EDT 1 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
    ASTM D 4175 : 2018 : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2016 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2016 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6974 : 2004 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
    ASTM D 7463 :2014 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated Water
    ASTM D 1129 : 2010 Standard Terminology Relating to Water
    ASTM E 1259 : 2018 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C
    ASTM D 1129 : 2006 : REV A : EDT 1 Standard Terminology Relating to Water
    ASTM D 1129 : 2001 Standard Terminology Relating to Water
    ASTM D 1129 : 2004 : EDT 1 Standard Terminology Relating to Water
    ASTM D 4175 : 2017 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 4175 : 2019 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2014 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM E 1259 : 2016 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
    ASTM D 4175 : 2015 : REV B : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
    ASTM E 1326 : 2013 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
    ASTM D 7464 : 2008 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
    ASTM D 4175 : 2008 : REV B Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
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