We noticed you’re not on the correct regional site. Switch to our AMERICAS site for the best experience.
  • ASTM D 8070 : 2021

    Current The latest, up-to-date edition.
    Add to Watchlist
    This Standard has been added successfully to your Watchlist.
    Please visit My Watchlist to see all standards that you are watching.
    Please log in or to add this standard to your Watchlist.
    We could not add this standard to your Watchlist.
    Please retry or contact support for assistance.
    You need to be logged in to add this standard to your Watchlist.
    Please log in now or create an account to add.
    You already added this Product in the Watchlist.

    Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay

    Available format(s):  Hardcopy, PDF

    Language(s):  English

    Published date:  20-04-2021

    Publisher:  American Society for Testing and Materials

    Add to Watchlist

    Add To Cart

    Scope - (Show below) - (Hide below)

    1.1This test method describes a procedure that can be used in the field or in a laboratory to detect antigens indicative of microbial contamination in liquid fuels, including those blended with synthesized hydrocarbons or biofuels, with kinematic viscosities (at 40 °C) of 24 mm2s–1 (for example, Specifications D396, D975, and D1655) and in fuel-associated water.

    1.1.1This test method has been validated by an ILS for a range of middle distillate fuels meeting Specification D1655, EN590, Specification D975, and ISO 8217:2012.

    1.2This test method semi-quantitatively assesses the concentration of specific antigens generated by aerobic microorganisms during active growth in fuels.

    1.2.1A proprietary formulation of antibodies and antibody mixtures is used to detect three types of microbial antigen contamination: antigens generally found in aerobic bacteria, antigens generally present in common fungi (yeast and molds), and an antigen that is characteristic of Hormoconis resinae (the fungus most commonly associated with fuel biodeterioration).

    1.2.2Although the antibodies and antibody mixtures are characteristic of diverse types of bacteria and fungi, it is unlikely that they are universal. Recognizing that for every microbe that has been isolated and characterized, it is likely that there are a billion that have not. Consequently, as is the case with all microbiological test methods, this test method does not purport to detect 100 % of the microbes present in a fuel or fuel-associated water sample.

    1.3For each of the three sets of antigen detected (H. resinae, common fungi, and aerobic bacteria), the test detects whether the antigen concentration present is within set ranges representing negligible, moderate, or heavy microbial contamination.

    1.3.1For fuel specimens, the antigen concentration ranges detected are <150 µg/L (negligible), 150 µg/L to 750 µg/L (moderate), and >750 µg/L (heavy).

    1.3.2For specimens of water associated with fuel, the antigen concentration ranges detected are <33 µg/mL (negligible), 33 µg/mL to 166 µg/mL (moderate), and >166 µg/mL (heavy).

    1.4The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.5This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For a specific hazard statement, see Section 8.

    1.6This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

    General Product Information - (Show below) - (Hide below)

    Committee D 02
    Document Type Test Method
    Publisher American Society for Testing and Materials
    Status Current
    Supersedes

    Standards Referenced By This Book - (Show below) - (Hide below)

    ASTM D 6469 : 2020 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
    ASTM D 7847 : 2017 Standard Guide for Interlaboratory Studies for Microbiological Test Methods

    Standards Referencing This Book - (Show below) - (Hide below)

    ASTM D 4176 : 2021 Standard Test Method for Free Water and Particulate Contamination in Distillate Fuels (Visual Inspection Procedures)
    ASTM D 4175 : 2020 : REV B Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 1655 : 2021 : REV A Standard Specification for Aviation Turbine Fuels
    ASTM D 1655 : 2020 : REV D Standard Specification for Aviation Turbine Fuels
    ASTM D 975 : 2021 Standard Specification for Diesel Fuel
    ASTM D 4175 : 2020 : REV B : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 975 : 2020 : REV C Standard Specification for Diesel Fuel
    ASTM D 1655 : 2021 : REV B Standard Specification for Aviation Turbine Fuels
    ASTM D 4176 : 2021 : REV A Standard Test Method for Free Water and Particulate Contamination in Distillate Fuels (Visual Inspection Procedures)
    ASTM D 4176 : 2020 Standard Test Method for Free Water and Particulate Contamination in Distillate Fuels (Visual Inspection Procedures)
    ASTM D 1655 : 2021 Standard Specification for Aviation Turbine Fuels
    ASTM D 1655 : 2021 : REV C Standard Specification for Aviation Turbine Fuels
    • Access your standards online with a subscription

      Features

      • Simple online access to standards, technical information and regulations
      • Critical updates of standards and customisable alerts and notifications
      • Multi - user online standards collection: secure, flexibile and cost effective