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  • ASTM D 8243 : 2019

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    Standard Test Method for Determination of APS Reductase to Estimate Sulfate Reducing Bacterial Bioburdens in Water – Enzyme-Linked Immunosorbent Assay Method

    Available format(s):  Hardcopy, PDF

    Language(s):  English

    Published date:  01-01-2019

    Publisher:  American Society for Testing and Materials

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    Abstract - (Show below) - (Hide below)

    1.1 This test method provides a protocol for using enzyme-linked immunosorbent assay (ELISA) technology to test water samples for the enzyme adenosine 5’-phosphosulfate reductase (APSr) concentration.

    Scope - (Show below) - (Hide below)

    1.1This test method provides a protocol for using enzyme-linked immunosorbent assay (ELISA) technology to test water samples for the enzyme adenosine 5’-phosphosulfate reductase (APSr) concentration.

    1.1.1APSr is present in all known sulfate reducing protists (SRP – sulfate reducing bacteria – SRB – and sulfate reducing archaea – SRA).

    1.1.2As reported in U.S. Patent 4,999,286, APS reductase concentration can be used as a surrogate parameter for estimating SRA bioburdens (Appendix X1 compares results from Test Methods D8243, D4412, and quantitative polymerase chain reaction – qPCR – testing).

    1.2This test method has been validated in tap water, oilfield produced water (salinities ranging from 100 g L-1 to 600 g L-1), and fuel-associated water (commonly referred to as bottoms-water).

    1.3This test method detects APS reductase semi-quantitatively in the range of 0.001M to 0.1M – correlating to 102 SRP/mL to 106 SRP/mL.

    1.3.1As described in Appendix X2 test method sensitivity can be increased 10-fold to 100-fold. However, the precision statistics provided in X apply only to 10-mL specimens.

    1.4The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.5This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Some specific hazards statements are given in Section 9 on Hazards.

    1.6This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

    General Product Information - (Show below) - (Hide below)

    Committee D 19
    Document Type Test Method
    Publisher American Society for Testing and Materials
    Status Current

    Standards Referencing This Book - (Show below) - (Hide below)

    ASTM D 6300 : 2020 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2013 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6300 : 2014 : REV A : EDT 1 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 7464 : 2020 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
    ASTM D 1129 : 2003 Standard Terminology Relating to Water
    ASTM D 6300 : 2017 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 1129 : 2013 Standard Terminology Relating to Water
    ASTM D 6499 : 2000 Standard Test Method for the Immunological Measurement of Antigenic Protein in Natural Rubber and its Products
    ASTM D 1129 : 2013 : R2020 Standard Terminology Relating to Water
    ASTM D 5847 : 1999 : REV A : EDT 1 Standard Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
    ASTM D 6300 : 2017 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 5847 : 2002 : R2007 Standard Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
    ASTM D 6300 : 2016 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6300 : 2004 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 1129 : 2006 Standard Terminology Relating to Water
    ASTM D 6499 : 2003 Standard Test Method for the Immunological Measurement of Antigenic Protein in Natural Rubber and its Products
    ASTM E 1847 : 1996 : R2008 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines
    ASTM E 1914 : 1998 Standard Practice for Use of Terms Relating to the Development and Evaluation of Methods for Chemical Analysis
    ASTM E 691 : 2020 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
    ASTM D 6300 : 2019 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 7464 : 2019 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
    ASTM D 5847 : 2002 : R2012 Standard Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
    ASTM D 6300 : 2021 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2019 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6300 : 2020 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 1129 : 1999 : REV A Standard Terminology Relating to Water
    ASTM E 691 : 2019 : EDT 1 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
    ASTM D 7464 : 2008 : R2013 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
    ASTM D 1129 : 2006 : REV A Standard Terminology Relating to Water
    ASTM E 1601 : 2019 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
    ASTM D 4412 : 2015 Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits
    ASTM D 5847 : 2002 : R2020 Standard Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
    ASTM D 1129 : 2003 : REV A Standard Terminology Relating to Water
    ASTM D 1129 : 2002 Standard Terminology Relating to Water
    ASTM E 1601 : 1998 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
    ASTM D 6300 : 2020 : REV B Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
    ASTM D 6300 : 2006 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM E 1847 : 1996 : R2003 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines
    ASTM D 6499 : 2018 Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products
    ASTM E 1601 : 2010 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
    ASTM D 6300 : 2014 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4412 : 1984 : R2009 Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits
    ASTM E 691 : 2022 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
    ASTM D 6300 : 2015 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6300 : 1999 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM E 691 : 2009 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
    ASTM E 1847 : 1996 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines
    ASTM E 1601 : 2012 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
    ASTM D 1129 : 2004 Standard Terminology Relating to Water
    ASTM D 5847 : 2022 Standard Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
    ASTM D 6499 : 2016 Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products
    ASTM D 6300 : 2013 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 7464 : 2014 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
    ASTM E 691 : 2021 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
    ASTM D 6499 : 2007 Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products
    ASTM D 6300 : 2008 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6300 : 2003 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 1129 : 2002 : REV A Standard Terminology Relating to Water
    ASTM D 6300 : 2007 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM E 1914 : 2007 Standard Practice for Use of Terms Relating to the Development and Evaluation of Methods for Chemical Analysis (Withdrawn 2016)
    ASTM D 4412 : 1984 : R2002 Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits
    ASTM D 5847 : 2002 Standard Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
    ASTM E 1847 : 1996 : R2013 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines (Withdrawn 2022)
    ASTM D 6300 : 2002 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 6499 : 2012 Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products
    ASTM D 6300 : 2016 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 1129 : 2010 Standard Terminology Relating to Water
    ASTM D 1129 : 2006 : REV A : EDT 1 Standard Terminology Relating to Water
    ASTM D 1129 : 2001 Standard Terminology Relating to Water
    ASTM D 1129 : 2004 : EDT 1 Standard Terminology Relating to Water
    ASTM D 6300 : 2014 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
    ASTM D 4412 : 1984 : R1997 Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits
    ASTM D 7464 : 2008 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
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