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  • ASTM E 1192 : 1997

    Superseded A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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    Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians

    Available format(s):  Hardcopy, PDF

    Superseded date:  11-11-2014

    Language(s):  English

    Published date:  10-02-1997

    Publisher:  American Society for Testing and Materials

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    Abstract - (Show below) - (Hide below)

    CONTAINED IN VOL. 11.06, 2014 Defines procedures for obtaining laboratory data concerning the adverse effects of an aqueous effluent on certain species of freshwater and saltwater fishes, macroinvertebrates, and amphibians, usually during 2 to 4-day exposures, depending on the species, using the static, renewal, and flow-through techniques.

    Scope - (Show below) - (Hide below)

    1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of an aqueous effluent on certain species of freshwater and saltwater fishes, macroinvertebrates, and amphibians usually during 2 to 4-day exposures, depending on the species, using the static, renewal, and flow-through techniques. These procedures will probably be useful for conducting acute toxicity tests on aqueous effluents with many other aquatic species, although modifications might be necessary.

    1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute toxicity tests on aqueous effluents.

    1.3 This guide is based in large part on Guide E 729. The major differences between the two guides are ( 1) the maximum test concentration is 100 % effluent or ambient sample, (2) testing is not chemical specific, and (3) the holding time of effluent and ambient samples is often considerably less that that for chemicals and other test materials. Because the sample is often a complex mixture of chemicals, analytical tests cannot generally be used to confirm exposure concentrations.

    1.4 Selection of the technique to be used in a specific situation will depend upon the needs of the investigator and upon available resources. Static tests provide the most easily obtained measure of acute toxicity, but should not last longer than 48 h. Renewal and flow-through tests may last longer than 48 h because the pH and concentrations of dissolved oxygen and effluent are maintained at desired levels and degradation and metabolic products are removed. Static tests might not be applicable to effluents that have a high oxygen demand, or contain materials that ( 1) are highly volatile, (2) are rapidly biologically or chemically transformed in aqueous solutions, or (3) are removed from test solutions in substantial quantities by the test chambers or organisms during the test. Flow-through tests are generally preferable to renewal tests, although in some situations a renewal test might be more cost-effective than a flow-through test.

    1.5 In the development of these procedures, an attempt was made to balance scientific and practical considerations and to ensure that the results will be sufficiently accurate and precise for the applications for which they are commonly used. A major consideration was that the common uses of the results of acute tests on effluents do not require or justify stricter requirements than those set forth in this guide. Although the tests may be improved by using more organisms, longer acclimation times, etc., the requirements presented in this guide should usually be sufficient.

    1.6 Results of acute toxicity tests should usually be reported in terms of a median lethal concentration (LC50) or median effective concentration (EC50). In some situations, it might be necessary only to determine whether a specific concentration is acutely toxic to the test species or whether the LC50 or EC50 is above or below a specific concentration.

    1.7 This guide is arranged as follows:

    9
    Section
    Referenced Documents2
    Terminology 3
    Summary of Guide4
    Significance and Use5
    Hazards7
    Apparatus 6
    Facilities 6.1
    Special Requirements6.2
    Construction Materials6.3
    Metering System6.4
    Test Chambers6.5
    Cleaning6.6
    Acceptability6.7
    Dilution Water 8
    Requirements8.1
    Source 8.2
    Treatment8.3
    Characterization 8.4
    Effluent
    Sampling Point 9.1
    Collection9.1
    Preservation 9.2
    Treatment9.3
    Test Concentration(s)
    Test Organisms
    Species
    Age
    Source
    Care and Handling
    Feeding
    Disease Treatment
    Holding
    Acclimation
    Quality
    Procedure
    Experimental Design
    Dissolved Oxygen
    Temperature
    Loading
    Beginning the Test
    Feeding
    Duration of Test
    Biological Data
    Other Measurements
    Analytical Methodology
    Acceptability of Test
    Calculation or Results
    Report

    1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section .

    General Product Information - (Show below) - (Hide below)

    Committee E 47
    Document Type Guide
    Product Note Reconfirmed 1997
    Publisher American Society for Testing and Materials
    Status Superseded
    Superseded By

    Standards Referenced By This Book - (Show below) - (Hide below)

    ASTM E 1218 : 2004 : R2012 Standard Guide for Conducting Static Toxicity Tests with Microalgae (Withdrawn 2021)
    ASTM E 2317 : 2004 : R2012 Standard Guide for Conducting Renewal Microplate-Based Life-Cycle Toxicity Tests with a Marine Meiobenthic Copepod (Withdrawn 2021)
    ASTM E 724 : 1998 : R2012 Standard Guide for Conducting Static Acute Toxicity Tests Starting with Embryos of Four Species of Saltwater Bivalve Molluscs
    ASTM E 1241 : 2005 : R2013 Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes (Withdrawn 2022)
    ASTM E 1498 : 1992 : R2012 Standard Guide for Conducting Sexual Reproduction Tests with Seaweeds (Withdrawn 2021)
    ASTM E 2122 : 2002 : R2013 Standard Guide for Conducting In-situ Field Bioassays With Caged Bivalves (Withdrawn 2022)
    ASTM E 1439 : 2012 : R2019 Standard Guide for Conducting the Frog Embryo Teratogenesis Assay-Xenopus (FETAX)
    ASTM E 1193 : 1997 : R2012 Daphnia magna
    ASTM E 1191 : 2003 : REV A : R2014 Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids
    ASTM E 1563 : 1998 : R2012 Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos
    ASTM E 729 : 1996 : R2014 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
    ASTM E 1841 : 2004 : R2012 Standard Guide for Conducting Renewal Phytotoxicity Tests With Freshwater Emergent Macrophytes (Withdrawn 2021)
    ASTM E 1440 : 1991 : R2012 Standard Guide for Acute Toxicity Test with the Rotifer Brachionus (Withdrawn 2021)
    ASTM E 1295 : 2001 : R2013 Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
    ASTM E 1850 : 2004 : R2019 Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests
    ASTM E 1611 : 2000 : R2013 Standard Guide for Conducting Sediment Toxicity Tests with Polychaetous Annelids
    ASTM E 1463 : 1992 : R2012 Standard Guide for Conducting Static and Flow-Through Acute Toxicity Tests With Mysids From the West Coast of the United States (Withdrawn 2021)
    ASTM E 1849 : 1996 : R2013 Standard Guide for Fish and Wildlife Incident Monitoring and Reporting (Withdrawn 2022)
    ASTM E 1768 : 1995 : R2013 Standard Guide for Ventilatory Behavioral Toxicology Testing of Freshwater Fish (Withdrawn 2022)
    ASTM E 1366 : 2011 : R2016 Standard Practice for Standardized Aquatic Microcosms: Fresh Water
    ASTM E 1415 : 1991 : R2012 Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3 (Withdrawn 2021)

    Standards Referencing This Book - (Show below) - (Hide below)

    ASTM E 1604 : 2012 : REDLINE Standard Guide for Behavioral Testing in Aquatic Toxicology
    IEEE/ASTM SI_10-2010 American National Standard for Metric Practice
    ASTM E 729 : 1996 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
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