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  • ASTM E 1218 : 2004 : R2012

    Withdrawn A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.
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    Standard Guide for Conducting Static Toxicity Tests with Microalgae (Withdrawn 2021)

    Available format(s):  Hardcopy, PDF

    Withdrawn date:  22-01-2021

    Language(s):  English

    Published date:  07-01-2013

    Publisher:  American Society for Testing and Materials

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    Abstract - (Show below) - (Hide below)

    CONTAINED IN VOL. 11.06, 2014 Describes procedures for obtaining laboratory data concerning the adverse effects of a test material added to growth medium on growth of certain species of freshwater and saltwater microalgae during a static exposure.

    Scope - (Show below) - (Hide below)

    1.1This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to growth medium on growth of certain species of freshwater and saltwater microalgae during a static exposure. These procedures will probably be useful for conducting short-term toxicity tests with other species of algae, although modifications might be necessary. Although the test duration is comparable to an acute toxicity test with aquatic animals, an algal toxicity test of short duration (72, 96 or 120 h) allows for examination of effects upon multiple generations of an algal population and thus should not be viewed as an acute toxicity test.

    1.2Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting toxicity tests with microalgae.

    1.3These procedures are applicable to many chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications, these procedures can be used to conduct tests on temperature, and pH and on such materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, sediments, and surface waters. Static tests might not be applicable to materials that are highly volatile, are rapidly biologically or chemically transformed in aqueous solutions, or are removed from test solutions in substantial quantities by the test vessels or organisms during the test. However, practical flow-through test procedures with microalgae have not been developed.

    1.4Results of tests using microalgae should usually be reported in terms of the 96-h (or other time period) IC50 (see 3.2.5) based on reduction in growth. In some situations, it might only be necessary to determine whether a specific concentration unacceptably affects the growth of the test species or whether the IC50 is above or below a specific concentration.

    1.5This guide is arranged as follows:

    Section

    Referenced Documents

    2

    Terminology

    3

    Summary of Guide

    4

    Significance and Use

    5

    Hazards

    7

    Apparatus

    6

     Facilities

    6.1

     Equipment

    6.2

     Test Vessels

    6.3

     Cleaning

    6.4

     Acceptability

    6.5

    Growth Medium

    8

    Test Material

    9

     General

    9.1

     Stock Solution

    9.2

     Test Concentration(s)

    9.3

    Test Organisms

    10

     Species

    10.1

     Source

    10.2

     Culture

    10.3

     Quality

    10.4

    Procedure

    11

     Experimental Design

    11.1

     Temperature

    11.2

     Illumination

    11.3

     Beginning the Test

    11.4

     Gas Exchange

    11.5

     Duration of Test

    11.6

     Biological Data

    11.7

     Other Measurements

    11.8

     Determination of Algistatic and Algicidal Effects

    11.8.5

    Analytical Methodology

    12

    Acceptability of Test

    13

    Calculation

    14

    Report

    15

    Keywords

    16


    1.6This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 7.

    General Product Information - (Show below) - (Hide below)

    Committee E 50
    Document Type Guide
    Product Note Reconfirmed 2012
    Publisher American Society for Testing and Materials
    Status Withdrawn
    Superseded By
    Supersedes

    Standards Referenced By This Book - (Show below) - (Hide below)

    ASTM E 1850 : 2004 : R2019 Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests
    ASTM E 2317 : 2004 : R2012 Standard Guide for Conducting Renewal Microplate-Based Life-Cycle Toxicity Tests with a Marine Meiobenthic Copepod (Withdrawn 2021)
    ASTM E 1841 : 2004 : R2012 Standard Guide for Conducting Renewal Phytotoxicity Tests With Freshwater Emergent Macrophytes (Withdrawn 2021)
    ASTM E 1415 : 1991 : R2012 Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3 (Withdrawn 2021)
    ASTM E 1733 : 1995 : R2014 Standard Guide for Use of Lighting in Laboratory Testing

    Standards Referencing This Book - (Show below) - (Hide below)

    ASTM E 1023 : 1984 : R2007 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
    ASTM D 3731 : 1987 : R2012 Standard Practices for Measurement of Chlorophyll Content of Algae in Surface Waters
    ASTM D 4447 : 2015 Standard Guide for Disposal of Laboratory Chemicals and Samples
    ASTM E 1733 : 1995 : R2014 Standard Guide for Use of Lighting in Laboratory Testing
    ASTM D 3978 : 2004 Practice for Algal Growth Potential Testing with Pseudokirchneriella subcapitata
    ASTM E 1733 : 1995 : R2002 : EDT 1 Standard Guide for Use of Lighting in Laboratory Testing
    ASTM E 380 : 1993 Standard Practice for Use of the Internation System of Units (SI) (the Modernized Metric System) (Withdrawn 1997)
    ASTM D 1129 : 2013 : REDLINE Standard Terminology Relating to Water
    ASTM D 1129 : 2003 Standard Terminology Relating to Water
    ASTM D 4447 : 1984 : R2003 Standard Guide for Disposal of Laboratory Chemicals and Samples
    ASTM E 1733 : 1995 Standard Guide for Use of Lighting in Laboratory Testing
    ASTM D 1129 : 2013 Standard Terminology Relating to Water
    ASTM D 1129 : 2013 : R2020 Standard Terminology Relating to Water
    ASTM D 3978 : 2004 : R2012 Standard Practice for Algal Growth Potential Testing with Pseudokirchneriella subcapitata
    ASTM D 4447 : 2015 : REDLINE Standard Guide for Disposal of Laboratory Chemicals and Samples
    ASTM D 4447 : 2006 Standard Guide for Disposal of Laboratory Chemicals and Samples
    ASTM E 1023 : 1984 : R2014 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
    ASTM D 1129 : 2006 Standard Terminology Relating to Water
    ASTM E 1847 : 1996 : R2008 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines
    ASTM E 1023 : 1984 : R2002 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
    ASTM D 3731 : 1987 : R1998 Standard Practices for Measurement of Chlorophyll Content of Algae in Surface Waters
    ASTM E 1192 : 1997 : R2008 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
    ASTM D 1129 : 1999 : REV A Standard Terminology Relating to Water
    ASTM D 1129 : 2006 : REV A Standard Terminology Relating to Water
    ASTM D 1129 : 2003 : REV A Standard Terminology Relating to Water
    ASTM D 1129 : 2002 Standard Terminology Relating to Water
    ASTM E 1023 : 1984 : R1996 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
    ASTM E 1847 : 1996 : R2003 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines
    ASTM E 1192 : 1997 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
    ASTM E 1847 : 1996 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines
    ASTM D 1129 : 2004 Standard Terminology Relating to Water
    ASTM D 4447 : 1984 : R1997 Standard Guide for Disposal of Laboratory Chemicals and Samples
    ASTM D 3731 : 1987 : R2004 Standard Practices for Measurement of Chlorophyll Content of Algae in Surface Waters
    ASTM E 1192 : 1997 : R2003 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
    ASTM D 1129 : 2002 : REV A Standard Terminology Relating to Water
    ASTM D 4447 : 2010 Standard Guide for Disposal of Laboratory Chemicals and Samples
    ASTM E 1847 : 1996 : R2013 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines (Withdrawn 2022)
    ASTM E 729 : 1996 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
    ASTM E 1192 : 1997 : R2014 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
    ASTM D 1129 : 2010 Standard Terminology Relating to Water
    ASTM D 1129 : 2006 : REV A : EDT 1 Standard Terminology Relating to Water
    ASTM D 1129 : 2001 Standard Terminology Relating to Water
    ASTM D 1129 : 2004 : EDT 1 Standard Terminology Relating to Water
    ASTM D 3978 : 1980 : R1998 Standard Practice for Algal Growth Potential Testing with Selenastrum Capricornutum
    ASTM E 1733 : 1995 : R2008 Standard Guide for Use of Lighting in Laboratory Testing
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