• There are no items in your cart
We noticed you’re not on the correct regional site. Switch to our AMERICAS site for the best experience.
Dismiss alert

ASTM E 1295 : 2001

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
Available format(s)

Hardcopy , PDF

Superseded date

11-11-2014

Language(s)

English

Published date

10-12-2001

CONTAINED IN VOL. 11.06, 2013 Defines procedures for obtaining data concerning the adverse effects of an effluent or a test material on Ceriodaphnia dubia Richard 1894, during continuous exposure throughout a portion of the organism's life.

1.1 This guide describes procedures for obtaining data concerning the adverse effects of an effluent or a test material (added to dilution water, but not to food) on Ceriodaphnia dubiaRichard 1894, during continuous exposure throughout a portion of the organism's life. These procedures should also be useful for conducting life cycle toxicity tests with other Cladocera (Guide E 1193), although modifications will be necessary.

1.2 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures, that can be measured accurately at the necessary concentrations in water. With appropriate modifications these procedures can be used to conduct tests on temperature, dissolved oxygen, pH, and on such materials as aqueous effluents (see also Guide E 1192), leachates, oils, particulate matter, sediments (see also Guide E 1383), and surface waters. Renewal tests might not be applicable to materials that have high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed, or sorb to test chambers. If the concentration of dissolved oxygen falls below 4 mg/L or the concentration of test material decreases by more than 20 % in test solution(s) between renewals, more frequent renewals might be necessary.

1.3 Other modifications of these procedures might be justified by special needs or circumstances. Results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information on new concepts and procedures for conducting three-brood toxicity tests with C. dubia.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 8.

1.4 This guide is arranged as follows:

Section
Referenced Documents2
Terminology3
Summary of Guide4
Significance and Use5
Apparatus6
Facilities6.1
Construction Materials6.2
Test Chambers6.3
Cleaning6.4
Reagents and Materials7
Hazards8
Dilution Water9
Requirements9.1
Source9.2
Treatment9.3
Characterization9.4
Test Material10
General10.1
Stock Solution10.2
Effluent10.3
Test Concentration(s)10.4
Collection10.5
Sample Containers10.6
Preservation10.7
Treatment10.8
Test Organisms11
Species11.1
Age11.2
Source11.3
Brood Stock11.4
Food11.5
Handling11.6
Quality11.7
Procedure12
Demonstration of Feasibility12.1
Experimental Design12.2
Dissolved Oxygen12.3
Temperature12.4
Preparing Test Solutions12.5
Conditioning Test Chambers12.6
Beginning a Test12.7
Renewing Test Solutions12.8
Duration of Test12.9
Biological Data12.10
Other Measurements12.11
Analytical Methodology13
Acceptability of Test14
Calculation15
Report16
Appendixes
FoodX1
Culture TechniquesX2
Test ChambersX3
Statistical GuidanceX4

Committee
E 47
DocumentType
Guide
Pages
20
ProductNote
Reconfirmed 2001
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

ASTM E 1849 : 1996 : R2013 Standard Guide for Fish and Wildlife Incident Monitoring and Reporting (Withdrawn 2022)
ASTM E 2172 : 2001 : R2014 Standard Guide for Conducting Laboratory Soil Toxicity Tests with the Nematode <i xmlns:a="http://library.astm.org" xmlns=""> Caenorhabditis elegans</i>
ASTM E 1022 : 1994 : R2013 Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks (Withdrawn 2022)
ASTM E 1706 : 2019 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates

IEEE/ASTM SI_10-2010 American National Standard for Metric Practice
ASTM E 729 : 1996 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians

View more information
£69.29
Excluding VAT

Access your standards online with a subscription

Features

  • Simple online access to standards, technical information and regulations.

  • Critical updates of standards and customisable alerts and notifications.

  • Multi-user online standards collection: secure, flexible and cost effective.