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  • ASTM E 1415 : 1991 : R2012

    Superseded A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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    Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3 (Withdrawn 2021)

    Available format(s):  Hardcopy, PDF

    Superseded date:  12-11-2022

    Language(s):  English

    Published date:  07-01-2013

    Publisher:  American Society for Testing and Materials

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    Abstract - (Show below) - (Hide below)

    CONTAINED IN VOL. 11.06, 2013 Specifies procedures for obtaining laboratory data concerning the adverse effects of a text material added to growth medium on a certain species of duckweed (Lemna gibba G3) during a 7-day exposure using the static technique.

    Scope - (Show below) - (Hide below)

    1.1This guide describes procedures for obtaining laboratory data concerning the adverse effects of a text material added to growth medium on a certain species of duckweed (Lemna gibba G3) during a 7-day exposure using the static technique. These procedures will probably be useful for conducting toxicity tests with other species of duckweed and other floating vascular plants, although modifications might be necessary.

    1.2Special needs or circumstances might also justify modification of this standard. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting tests with duckweed.

    1.3The procedures in this guide are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications these procedures can be used to conduct tests on temperature and pH and on such other materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, sediments and surface waters. These procedures do not specifically address effluents because to date there is little experience using duckweeds in effluent testing and such tests may pose problems with acclimation of the test organisms to the receiving water. Static tests might not be applicable to materials that have a high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed in aqueous solution, or are removed from test solutions in substantial quantities by the test chambers or organisms during the test.

    1.4Results of toxicity tests performed using the procedures in this guide should usually be reported in terms of the 7-day IC50 based on inhibition of growth. In some situations it might only be necessary to determine whether a specific concentration unacceptably affects the growth of the test species or whether the IC50 is above or below a specific concentration. Another end point that may be calculated is the no observed effect concentration (NOEC).

    1.5The sections of this guide appear as follows:

      Title

    Section

    Referenced Documents

    2

    Terminology

    3

    Summary of Guide

    4

    Significance and Use

    5

    Hazards

    6

    Apparatus

    7

     Facilities

    7.1

     Test Chambers

    7.2

     Cleaning

    7.3

     Acceptability

    7.4

    Growth Medium

    8

     Test Material

    9

     General

    9.1

     Stock Solution

    9.2

     Test Concentration(s)

    9.3

    Test Organisms

    10

     Species

    10.1

     Source

    10.2

     Stock Culture

    10.3

    Procedure

    11

     Experimental Design

    11.1

     Temperature

    11.2

     Illumination

    11.3

     Beginning the Test

    11.4

     Duration of Test

    11.5

     Biological Data

    11.6

     Other Measurements

    11.7

    Analytical Methodology

    12

    Acceptability of Test

    13

    Calculation of Results

    14

    Report

    15


    1.6This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and to determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 6.

    General Product Information - (Show below) - (Hide below)

    Committee E 50
    Document Type Guide
    Product Note Reconfirmed 2012
    Publisher American Society for Testing and Materials
    Status Superseded
    Superseded By
    Supersedes

    Standards Referenced By This Book - (Show below) - (Hide below)

    ASTM E 1733 : 1995 : R2014 Standard Guide for Use of Lighting in Laboratory Testing
    ASTM E 2552 : 2016 Standard Guide for Assessing the Environmental and Human Health Impacts of New Compounds for Military Use
    ASTM E 1850 : 2004 : R2019 Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests
    ASTM E 1733 : 2022 Standard Guide for Use of Lighting in Laboratory Testing

    Standards Referencing This Book - (Show below) - (Hide below)

    ASTM E 1023 : 1984 : R2007 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
    ASTM E 1023 : 1984 : R2014 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
    ASTM E 1023 : 1984 : R2002 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
    ASTM E 1192 : 1997 : R2008 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
    IEEE/ASTM SI_10-2010 American National Standard for Metric Practice
    ASTM E 1218 : 2004 : R2012 Standard Guide for Conducting Static Toxicity Tests with Microalgae (Withdrawn 2021)
    ASTM E 1218 : 2004 Standard Guide for Conducting Static Toxicity Tests with Microalgae
    ASTM E 1023 : 1984 : R1996 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
    ASTM E 1192 : 1997 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
    ASTM E 1218 : 1997 : REV A Standard Guide for Conducting Static 96-h Toxicity Tests with Microalgae
    ASTM E 1192 : 1997 : R2003 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
    ASTM E 729 : 1996 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
    ASTM E 1192 : 1997 : R2014 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
    ASTM E 1218 : 2004 : EDT 1 Standard Guide for Conducting Static Toxicity Tests with Microalgae
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