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  • ASTM E 1563 : 1998 : R2012

    Superseded A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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    Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos

    Available format(s):  Hardcopy, PDF

    Superseded date:  11-02-2021

    Language(s):  English

    Published date:  09-01-2013

    Publisher:  American Society for Testing and Materials

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    Abstract - (Show below) - (Hide below)

    CONTAINED IN VOL. 11.06, 2015 Explains procedures for obtaining laboratory data concerning the acute effects of a test material on embryos and the resulting larvae of echinoid embryos (sea urchins and sand dollars) during static 48- to 96-h exposures.

    Scope - (Show below) - (Hide below)

    1.1This guide covers procedures for obtaining laboratory data concerning the acute effects of a test material on embryos and the resulting larvae of echinoid embryos (sea urchins and sand dollars) during static 48- to 96-h exposures. These procedures have generally been used with U.S. East Coast (Arbacia punctulata and Strongylocentrotus droebachiensis ) (1)3 and West Coast species (Strongylocentrotus purpuratus, S. droebachiensis, and Dendraster excentricus) (2). The basic procedures described in this guide first originated in Japan and Scandanavia (3), and parallel procedures have been used with foreign species, especially in Japan and the Mediterranean (4). These procedures will probably be useful for conducting static acute toxicity tests with embryos of other echinoid species, although modifications might be necessary.

    1.2Other modifications of these procedures might be justified by special needs or circumstances. Although using procedures appropriate to a particular species or special needs and circumstances is more important than following prescribed procedures, the results of tests conducted by using unusual procedures are not likely to be comparable with those of many other tests. The comparison of results obtained by using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute tests starting with embryos of echinoids.

    1.3These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications, these procedures can be used to conduct acute tests on temperature, dissolved oxygen, and pH and on such materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, surface waters and sediments (Annex A1). Renewal tests might be preferable to static tests for materials that have a high oxygen demand, are highly volatile, are rapidly transformed biologically or chemically in aqueous solution, or are removed from test solutions in substantial quantities by the test chambers or organisms during the test.

    1.4Results of acute toxicity tests with echinoid embryos should usually be reported as the 50 % effect concentration (EC50) based on the total abnormally developed embryos and larvae. In some situations, it might only be necessary to determine whether a specific concentration is acutely toxic to embryos or whether the EC50 is above or below a specific concentration.

    1.5This guide is arranged as follows:


    Section

    Scope

    1

    Referenced Documents

    2

    Terminology

    3

    Summary of Guide

    4

    Significance and Use

    5

    Apparatus

    6

     Facilities

    6.1

     Construction Materials

    6.2

     Test Chambers

    6.3

     Cleaning

    6.4

     Acceptability

    6.5

    Safety Precautions

    7

    Dilution Water

    8

     Requirements

    8.1

     Source

    8.2

     Treatment

    8.3

     Characterization

    8.4

    Test Material

    9

     General

    9.1

     Stock Solution

    9.2

     Test Concentration(s)

    9.3

    Test Organisms

    10

     Species

    10.1

     Age

    10.2

     Source of Embryos

    10.3

     Handling

    10.4

     Test Animal Source and Condition

    10.5

     Spawning and Fertilization

    10.6

     Quality

    10.7

    Procedure

    11

     Experimental Design

    11.1

     Dissolved Oxygen

    11.2

     Temperature

    11.3

     Beginning the Test

    11.4

     Feeding

    11.5

     Duration of Test

    11.6

     Biological Data

    11.7

     Control Performance

    11.8

     Other Measurements

    11.9

    Analytical Methods

    12

    Acceptability of Test

    13

    Calculation of Results

    14

    Report

    15

    Keywords

    16

    Annex

     Sediment Tests

    Annex A1




    1.6The values stated in SI units are to be regarded as the standard.

    1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations. Specific precautionary statements are given in Section 7.

    General Product Information - (Show below) - (Hide below)

    Committee E 50
    Document Type Guide
    Product Note Reconfirmed 2012
    Publisher American Society for Testing and Materials
    Status Superseded
    Superseded By
    Supersedes

    Standards Referenced By This Book - (Show below) - (Hide below)

    ASTM E 1525 : 2002 : R2014 Standard Guide for Designing Biological Tests with Sediments
    ASTM E 729 : 1996 : R2014 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
    ASTM E 1850 : 2004 : R2019 Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests

    Standards Referencing This Book - (Show below) - (Hide below)

    ASTM E 1367 : 2003 : EDT 1 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
    ASTM E 1023 : 1984 : R2007 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
    ASTM E 1391 : 2002 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing
    ASTM E 1706 : 2005 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
    ASTM E 380 : 1993 Standard Practice for Use of the Internation System of Units (SI) (the Modernized Metric System) (Withdrawn 1997)
    ASTM E 1391 : 2003 : R2014 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
    ASTM E 1525 : 1994 : REV A Standard Guide for Designing Biological Tests with Sediments
    ASTM E 1023 : 1984 : R2014 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
    ASTM E 1367 : 2003 : R2014 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
    ASTM E 1023 : 1984 : R2002 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
    ASTM E 1706 : 2000 : EDT 1 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Fresh Water Invertebrates
    ASTM E 1706 : 2020 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
    ASTM E 1391 : 1994 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing
    ASTM E 1391 : 2003 : R2008 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
    ASTM E 1706 : 2000 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Fresh Water Invertebrates
    ASTM E 1706 : 2005 : EDT 1 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
    ASTM E 1192 : 1997 : R2008 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
    ASTM E 1367 : 2003 : R2008 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
    ASTM E 1023 : 1984 : R1996 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
    ASTM E 1367 : 1992 Standard Guide for Conducting 10-day Static Sediment Toxicity Tests with Marine and Estuarine Amphipods
    ASTM E 1391 : 2003 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
    ASTM E 1192 : 1997 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
    ASTM E 1367 : 2003 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
    ASTM E 1706 : 2005 : R2010 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates (Withdrawn 2019)
    ASTM E 1525 : 2002 : R2008 Standard Guide for Designing Biological Tests with Sediments
    ASTM E 1706 : 2000 : EDT 2 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
    ASTM E 1192 : 1997 : R2003 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
    ASTM E 1706 : 2004 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
    ASTM E 729 : 1996 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
    ASTM E 1192 : 1997 : R2014 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
    ASTM E 1706 : 2019 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
    ASTM E 1525 : 2002 Standard Guide for Designing Biological Tests with Sediments
    ASTM E 1367 : 1999 Standard Guide for Conducting 10-day Static Sediment Toxicity Tests with Marine and Estuarine Amphipods
    ASTM E 1525 : 2002 : R2014 Standard Guide for Designing Biological Tests with Sediments
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