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ASTM E 2526 : 2008 : R2013

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Test Method for Evaluation of Cytotoxicity of Nanoparticulate Materials in Porcine Kidney Cells and Human Hepatocarcinoma Cells (Withdrawn 2022)
Available format(s)

Hardcopy , PDF

Superseded date

25-07-2022

Language(s)

English

Published date

07-10-2013

Publisher

American Society for Testing and Materials

CONTAINED IN VOL. 14.02, 2015 Defines a methodology to assess the cytotoxicity of suspensions of nanoparticulate materials in porcine proximal tubule cells (LLC-PK1) and human hepatocarcinoma cells (Hep G2) which represents potential target organs following systemic administration.

1.1This test method provides a methodology to assess the cytotoxicity of suspensions of nanoparticulate materials in porcine proximal tubule cells (LLC-PK1) and human hepatocarcinoma cells (Hep G2) which represents potential target organs following systemic administration

1.2This test method is part of the in vitro preclinical characterization cascade.

1.3This test method consists of a protocol utilizing two methods for estimation of cytotoxicity, 3-(4,5-Dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release.

1.4The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.5This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Committee
E 56
DocumentType
Test Method
Pages
6
ProductNote
Reconfirmed 2013
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

ASTM F 1877 : 2016 : REDLINE Standard Practice for Characterization of Particles
ASTM F 1877 : 2005 Standard Practice for Characterization of Particles
ASTM F 1903 : 2010 Standard Practice for Testing For Biological Responses to Particles <i>In Vitro</i>
ASTM F 1903 : 2010-07 PRACTICE FOR TESTING FOR BIOLOGICAL RESPONSES TO PARTICLES IN VITRO
ASTM F 1903 : 1998 Standard Practice for Testing for Biological Responses to Particles In Vitro
ASTM F 1903 : 2018 Standard Practice for Testing for Cellular Responses to Particles <emph type="bdit" >in vitro</emph>
ASTM F 1877 : 2016 Standard Practice for Characterization of Particles
ASTM F 1877 : 1998 Standard Practice for Characterization of Particles
ASTM F 1877 : 2005 : R2010 Standard Practice for Characterization of Particles
ASTM F 1877 : 2005 : EDT 1 Standard Practice for Characterization of Particles

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Hardcopy - English
PDF - English
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