1.1This test method specifies the operational parameters required to perform a quantitative liquid disinfectant efficacy test against biofilm bacteria.

1.2The test method was developed using a Pseudomonas aeruginosa biofilm grown in the CDC Biofilm Reactor (Test Method E2562), modified to include borosilicate glass coupons as a hard nonporous surface and P. aeruginosa ATCC 15442.

1.3Disinfectant preparation and contact time are used in the assessment according to the manufacturer’s instructions for use.

1.4The test method uses a closed system to treat biofilm. A coupon is placed in a single tube for the treatment, neutralization, and sampling steps to prevent the loss of cells.

1.5Verification of disinfectant neutralization is determined prior to conducting the test method.

1.6This test method describes how to sample and analyze treated and untreated control biofilms for viable cells. Biofilm population density is recorded as log₁₀ colony-forming units per coupon. Efficacy is reported as a log₁₀ reduction of viable cells.

1.7Basic microbiology training is required to perform this assay.

1.8The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.9This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.