Foodstuffs. Determination of vitamin E by high performance liquid chromatography. Measurement of α-, β-, γ- and δ-tocopherol

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

30-06-2014

Publisher

British Standards Institution

Foreword
Introduction
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Procedure
8 Calculation
9 Precision
10 Test report
Annex A (informative) - Examples of HPLC
chromatograms
Annex B (informative) - Precision data
Annex C (informative) - Alternative HPLC systems
Bibliography

Describes a method for the determination of Vitamin E in foodstuffs by high performance liquid chromatography (HPLC).

This European Standard specifies a method for the determination of vitamin E in foods by high performance liquid chromatography (HPLC). The determination of vitamin E content is carried out by measurement of α-, β-, γ- and δ-tocopherol. This method has been validated in two interlaboratory studies. The first study was for the analysis of α-tocopherol in margarine and milk powder ranging from 9,89 mg/100 g to 24,09 mg/100 g. The second study was for the analysis of α-, β-, γ- and δ-tocopherol in milk powder and of α-, and β-tocopherol in oat powder ranging from 0,057 mg/100 g (β-tocopherol) to 10,2 mg/100 g (α-tocopherol).

NOTE The vitamin E activity can be calculated from the tocopherol content assuming appropriate factors as given in [1], [2], [3] and [4].

Committee	AW/275
DevelopmentNote	Supersedes 97/707077 DC. (05/2005) Supersedes 12/30258770 DC. (06/2014)
DocumentType	Standard
Pages	24
PublisherName	British Standards Institution
Status	Current
Supersedes	BS EN 12822:2000 97/707077 DC : MAY 97 12/30258770 DC : 0

Standards	Relationship
NBN EN 12822 : 2014	Identical
I.S. EN 12822:2014	Identical
UNI EN 12822 : 2014	Identical
UNE-EN 12822:2014	Identical
NEN EN 12822 : 2014	Identical
EN 12822:2014	Identical
NF EN 12822 : 2014	Identical
SN EN 12822:2014	Identical
DIN EN 12822:2014-08	Identical
NS EN 12822 : 2014	Identical
DIN EN 12822:2000-07	Identical

ISO/IEC 17025:2005	General requirements for the competence of testing and calibration laboratories
ISO 5725:1986	Precision of test methods Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests
ISO 3696:1987	Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995	Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

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BS EN 12822:2014

Foodstuffs. Determination of vitamin E by high performance liquid chromatography. Measurement of α-, β-, γ- and δ-tocopherol

Table of Contents

Abstract

Scope

General Product Information

International Equivalents

Standards Referencing This Book

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