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BS EN 14122:2014

Current
Current

The latest, up-to-date edition.

Foodstuffs. Determination of vitamin B1 by high performance liquid chromatography
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

30-06-2014

Publisher

British Standards Institution

Foreword
1 Scope
2 Normative references
3 Principles
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) - Examples of HPLC chromatograms
Annex B (informative) - Precision data
Annex C (informative) - Alternative HPLC systems
Annex D (informative) - Vitamin B1 compound: 2-
        (1-hydroxyethyl)thiamin (HET) performing
        post-column derivatization
Bibliography

Defines a method for the determination of vitamin B[1] in food by high performance liquid chromatography (HPLC) with enzymatic treatment and pre- or post-column derivatization.

This European Standard specifies a method for the determination of vitamin B1 in food by high performance liquid chromatography (HPLC) with enzymatic treatment and pre- or post-column derivatization. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of whole meal flour, milk powder/spray dried milk, freeze-dried mixed vegetables and freeze-dried pig\'s liver ranging from 0,295 mg/100 g to 0,807 mg/100 g. The second study was for the analysis of samples of tube feeding solution, baby food with vegetables, powdered milk, meal with fruits, yeast, cereal, chocolate powder and food supplement ranging from 0,11 mg/100 g to 486 mg/100 g. Vitamin B1 is the mass fraction of total thiamin including its phosphorylated derivatives.

For further information on the validation, see Clause 8 and Annex B.

Committee
AW/275
DevelopmentNote
Supersedes 01/705156 DC. (06/2003) Supersedes 12/30258761 DC. (06/2014)
DocumentType
Standard
Pages
26
PublisherName
British Standards Institution
Status
Current
Supersedes

Standards Relationship
NEN EN 14122 : 2014 Identical
EN 14122:2014 Identical
NF EN 14122 : 2014 Identical
I.S. EN 14122:2014 Identical
NBN EN 14122 : 2014 Identical
UNE-EN 14122:2014 Identical
UNI EN 14122 : 2014 Identical
NS EN 14122 : 2014 Identical
SN EN 14122 : 2014 Identical
DIN EN 14122:2014-08 Identical
DIN EN 14122:2003-09 Identical

12/30258579 DC : 0 BS 5908-1 - FIRE AND EXPLOSION PRECAUTIONS AT PREMISES HANDLING FLAMMABLE GASES, LIQUIDS AND DUSTS - PART 1: CODE OF PRACTICE FOR PRECAUTIONS AGAINST FIRE AND EXPLOSION IN CHEMICAL PLANTS, CHEMICAL STORAGE AND SIMILAR PREMISES
BS 5908-1:2012 Fire and explosion precautions at premises handling flammable gases, liquids and dusts Code of practice for precautions against fire and explosion in chemical plants, chemical storage and similar premises
06/30124387 DC : DRAFT MAR 2006 BS 6349-8 - MARITIME STRUCTURES - PART 8: CODE OF PRACTICE FOR THE DESIGN OF RO-RO RAMPS, LINKSPANS AND WALKWAYS

ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

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