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BS EN ISO 21872-1:2017

Withdrawn
Withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

Microbiology of the food chain. Horizontal method for the determination of Vibrio spp. Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus
Amended by

BS EN ISO 21872-1:2017+A1:2023

Available format(s)

Hardcopy , PDF

Withdrawn date

16-03-2023

Language(s)

English

Published date

22-09-2017

Publisher

British Standards Institution

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Culture media and reagents
6 Equipment and consumables
7 Sampling
8 Preparation of the test sample
9 Procedure
10 Expression of results
11 Performance characteristics of the method
12 Test report
Annex A (normative) - Diagram of procedure
Annex B (normative) - Composition and preparation
        of the culture media and reagents
Annex C (informative) - Conventional PCR for the
        detection of Vibrio parahaemolyticus,
        thermostable direct haemolysin (tdh) and
        thermostable direct related haemolysin (trh)
        genes, Vibrio cholerae and Vibrio vulnificus
Annex D (informative) - Real-time PCR for the
        detection of Vibrio parahaemolyticus,
        thermostable direct haemolysin gene (tdh)
        and Vibrio vulnificus
Annex E (informative) - Results of an interlaboratory
        study
Bibliography

Defines a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract.

This document specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus. It is applicable to the following: products intended for human consumption and the feeding of animals; environmental samples in the area of food production and food handling. NOTE1 This method may not be appropriate in every detail for certain products (see Introduction). NOTE2 The World Health Organization (WHO) has identified that V. parahaemolyticus, V. cholerae and V. vulnificus are the major food-borne Vibrio spp. However, the method in this document can also be appropriate for the identification of other Vibrio spp. causing illness in humans. [1]

Committee
AW/9
DevelopmentNote
Supersedes DD ISO/TS 21872-1. (10/2017)
DocumentType
Standard
Pages
44
PublisherName
British Standards Institution
Status
Withdrawn
Supersedes
UnderRevision

Standards Relationship
ISO 21872-1:2017 Identical
EN ISO 21872-1:2017 Identical

ISO 22174:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 6887-1:2017 Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO/TS 20836:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Performance testing for thermal cyclers
ISO 20837:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for sample preparation for qualitative detection
ISO 22118:2011 Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics
ISO 20838:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 11133:2014 Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 6887-3:2017 Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products
ISO 17468:2016 Microbiology of the food chain — Technical requirements and guidance on establishment or revision of a standardized reference method
ISO 22119:2011 Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions

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PDF - English
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