Name: CEN/TR 15215-2:2006
Brand: CEN

Characterization of sludges - Detection and enumeration of Salmonella spp. in sludges, soils, soil improvers, growing media and biowastes - Part 2: Liquid enrichment method in selenite-cystine medium followed by Rapport-Vassiliadis for semi-quantitative Most Probable Number (MPN) determination

Published date

25-01-2006

Publisher

Comite Europeen de Normalisation

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus
6 Sampling and hazards
7 Reagents, diluents and culture media
8 Procedure
9 Expression of results
10 Test Report
11 Performance data
Annex A (normative) Most Probable Number in 100 ml of
        non-diluted suspension (8.1.2) = 10 g dry
        residue (combination 10 ml - 1 ml - 0,1 ml)
Annex B (informative) Method performance Characterisation
        of Salmonella spp. Enumeration method
Bibliography

This part of the CEN Technical Report method describes a method to detect and semi-quantitatively determine Salmonellae in sludges, soils, soil improvers, growing media and biowastes in accordance with the requirements of the European Sewage Sludge Regulation Revision of Directive 86/278/EEC (3rd Draft, CEN/TC 308 - doc525).The fully defined scope will be determined after the proposed validation trials have been agreed and carried out. The method has a limit of detection of approximately 1cfu/g wet weight sample.NOTEThe objective is to cover untreated and treated sludges, soils, soil improvers, growing media, biowastes and associated materials.

Committee	CEN/TC 308
DocumentType	Technical Report
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
FD CEN/TR 15215-2 : 2006 FD	Identical
I.S. CEN/TR 15215-2:2006	Identical

ISO 5667-13:2011	Water quality Sampling Part 13: Guidance on sampling of sludges
DIN 38414-13:1992-03	GERMAN STANDARD METHODS FOR THE EXAMINATION OF WATER, WASTE WATER AND SLUDGE; SLUDGE AND SEDIMENTS (GROUP S); DETECTION OF SALMONELLAE IN DISINFECTED SEWAGE SLUDGES (S 13)
EN 12880:2000	Characterization of sludges - Determination of dry residue and water content
ISO 8199:2005	Water quality General guidance on the enumeration of micro-organisms by culture
EN ISO 5667-13:2011	Water quality - Sampling - Part 13: Guidance on sampling of sludges (ISO 5667-13:2011)
EN 1040:2005	Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics - Test method and requirements (phase 1)