Name: DIN EN 16418 E : 2014
Brand: DIN

PAPER AND BOARD - DETERMINATION OF THE CYTOTOXICITY OF AQUEOUS EXTRACTS USING A METABOLICALLY COMPETENT HEPATOMA CELL LINE (HEPG2)

Published date

30-09-2014

Publisher

German Institute for Standardisation (Deutsches Institut für Normung)

National foreword National Annex NA (informative) - Bibliography Foreword 1 Scope 2 Normative references 3 Terms and definitions 4 Principle 5 Reagents 6 Cell line 7 Food simulants used for testing 8 Cleaning laboratory glassware 9 Equipment 10 Preparation of specimens 11 Cytotoxicity assessment 12 Expression of the results 13 Interpretation of the results 14 Precision 15 Test report Annex A (informative) - 96-well plates configuration Annex B (informative) - RNA Synthesis rate inhibition         cytotoxicity test work flow Annex C (informative) - Validation of the two methods         (option A and B) Bibliography

Defines a test method for the laboratory assessment of the potential cytotoxic effect of paper and board intended to come into contact with foodstuffs using specifically the HepG2 cell line.

DocumentType	Standard
PublisherName	German Institute for Standardisation (Deutsches Institut für Normung)
Status	Current

Standards	Relationship
EN 16418:2014	Identical

EN 645:1993	Paper and board intended to come into contact with foodstuffs - Preparation of a cold water extract
EN 15845:2010	Paper and board - Determination of the cytotoxicity of aqueous extracts
ISO 3696:1987	Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995	Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
EN 647:1993	Paper and board intended to come into contact with foodstuffs - Preparation of a hot water extract
DIN ISO 3696:1991-06	WATER FOR ANALYTICAL LABORATORY USE - SPECIFICATION AND TEST METHODS