Name: EN 15835:2010
Brand: CEN

Foodstuffs - Determination of ochratoxin A in cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection

Published date

03-02-2010

Publisher

Comite Europeen de Normalisation

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 HPLC analysis
8 Calculation
9 Precision
10 Test report
Annex A (informative) Typical chromatograms
Annex B (informative) Precision data
Annex C (informative) Data on the in-house study
Bibliography

This European Standard specifies a method for the determination of ochratoxin A in cereal based foods for infants and young children by high performance liquid chromatography (HPLC) with immunoaffinity column cleanup and fluorescence detection. This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 0,050 µg/kg to 0,217 µg/kg. For further information on the validation see Clause 8 and Annex B. Additional studies have shown that this method is applicable to cereal based baby foods containing 8 different types of cereals, honey and cocoa, at levels up to 3,540 µg/kg, see Annex C and [6].

Committee	CEN/TC 275
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
NBN EN 15835 : 2010	Identical
I.S. EN 15835:2010	Identical
DIN EN 15835:2010-05	Identical
PN EN 15835 : NOV 2010	Identical
BS EN 15835:2010	Identical
NS EN 15835 : 2010	Identical
NEN EN 15835 : 2010	Identical
DIN EN 15835 E : 2010	Identical
SN EN 15835 : 2010	Identical
UNI EN 15835 : 2010	Identical
NF EN 15835 : 2010	Identical
UNE-EN 15835:2010	Identical

ISO 5725-6:1994	Accuracy (trueness and precision) of measurement methods and results — Part 6: Use in practice of accuracy values
ISO 5725-4:1994	Accuracy (trueness and precision) of measurement methods and results Part 4: Basic methods for the determination of the trueness of a standard measurement method
ISO 5725-2:1994	Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 3696:1987	Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995	Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)