Name: EN ISO 20227:2017
Brand: CEN

Water quality - Determination of the growth inhibition effects of waste waters, natural waters and chemicals on the duckweed Spirodela polyrhiza - Method using a stock culture independent microbiotest (ISO 20227:2017)

Published date

05-07-2017

Publisher

Comite Europeen de Normalisation

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test organisms
6 Growth medium
7 Apparatus
8 Reference chemicals
9 Procedure
10 Data treatment - Calculation of the growth inhibition
11 Validity criterion
12 Test sensitivity
13 Test with reference chemicals
14 Test report
Annex A (informative) - Spirodela polyrhiza stock
culturing for turion production
Annex B (informative) - Sensitivity of the Spirodela
polyrhiza microbiotest
Annex C (informative) - Performance data
Bibliography

ISO 20227:2017 specifies a method for the determination of the inhibition of the growth of the first fronds of Spirodela polyrhiza germinated from turions, by substances and mixtures contained in water or waste water, including treated municipal waste water and industrial effluents.The test is also applicable to pure chemicals and in particular, plant protection products and pesticides.

Committee	CEN/TC 230
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
SS-EN ISO 20227:2017	Identical
ISO 20227:2017	Identical
PN EN ISO 20227 : 2017	Identical
I.S. EN ISO 20227:2017	Identical
DIN EN ISO 20227:2016-06 (Draft)	Identical
UNE-EN ISO 20227:2018	Identical
SN EN ISO 20227 : 2017	Identical
NF EN ISO 20227 : 2017	Identical
BS EN ISO 20227:2017	Identical
NEN EN ISO 20227 : 2017	Identical
NS EN ISO 20227 : 2017	Identical
UNI EN ISO 20227 : 2017	Identical
DIN EN ISO 20227 E : 2017	Identical
DIN EN ISO 20227:2017-10	Identical

ISO 5725-1:1994	Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 20079:2005	Water quality Determination of the toxic effect of water constituents and waste water on duckweed (Lemna minor) Duckweed growth inhibition test
NFT 90 337 : 96 XP	TESTING WATER - DETERMINATION OF THE INHIBITORY EFFECT ON THE GROWTH OF LEMNA MINOR
ISO 5725-2:1994	Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO/TS 20281:2006	Water quality — Guidance on statistical interpretation of ecotoxicity data
ISO 19827:2016	Water quality Determination of the acute toxicity to the freshwater rotifer Brachionus calyciflorus
ISO 5667-16:2017	Water quality — Sampling — Part 16: Guidance on biotesting of samples