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EN ISO 21149:2017

Current
Current

The latest, up-to-date edition.

Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria (ISO 21149:2017)
Published date

21-06-2017

European Foreword
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Diluents, neutralizers and culture media
6 Apparatus and glassware
7 Strains of microorganisms
8 Handling of cosmetic products and laboratory samples
9 Procedure
10 Counting of colonies (plate counts and membrane
   filtration methods)
11 Detection of growth (enrichment method)
12 Expression of results
13 Neutralization of the antimicrobial properties of
   the product
14 Test report
Annex A (informative) - Other neutralizing diluents
Annex B (informative) - Other diluents
Annex C (informative) - Other culture media
Annex D (informative) - Neutralizers of antimicrobial
        activity of preservatives and rinsing liquids
Bibliography

ISO 21149:2017 gives general guidelines for enumeration and detection of aerobic mesophilic bacteria present in cosmetics- by counting the colonies on agar medium after aerobic incubation, or- by checking the absence of bacterial growth after enrichment.Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.If needed, microorganisms enumerated or detected may be identified using suitable identification tests described in the standards given in the Bibliography.In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which this document is applicable. Products considered to present a low microbiological risk (see ISO 29621) include those with low water activity, hydro-alcoholic products, extreme pH values, etc.

Committee
CEN/TC 392
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

ISO 29621:2017 Cosmetics — Microbiology — Guidelines for the risk assessment and identification of microbiologically low-risk products
ISO 22718:2015 Cosmetics Microbiology Detection of Staphylococcus aureus
EN 12353:2013 Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal (including Legionella), mycobactericidal, sporicidal, fungicidal and virucidal (including bacteriophages) activity
ISO 22717:2015 Cosmetics Microbiology Detection of Pseudomonas aeruginosa
ISO 18415:2017 Cosmetics — Microbiology — Detection of specified and non-specified microorganisms
ISO 18416:2015 Cosmetics Microbiology Detection of Candida albicans
ISO 21148:2017 Cosmetics — Microbiology — General instructions for microbiological examination
EN 1040:2005 Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics - Test method and requirements (phase 1)
ISO 21150:2015 Cosmetics Microbiology Detection of Escherichia coli

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