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  • I.S. CEN/TR 15214-2:2006

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    CHARACTERIZATION OF SLUDGES - DETECTION AND ENUMERATION OF ESCHERICHIA COLI IN SLUDGES, SOILS, SOIL IMPROVERS, GROWING MEDIA AND BIOWASTES - PART 2: MINIATURISED METHOD (MOST PROBABLE NUMBER) BY INOCULATION IN LIQUID MEDIUM

    Available format(s):  Hardcopy, PDF

    Language(s):  English

    Published date:  01-01-2006

    Publisher:  National Standards Authority of Ireland

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    Table of Contents - (Show below) - (Hide below)

    Foreword
    Introduction
    1 Scope
    2 Normative references
    3 Terms and definitions
    4 Principle
    5 Apparatus
    6 Sampling and hazards
       6.1 Introduction
       6.2 General
       6.3 Storage
       6.4 Handling
    7 Reagents, diluents and culture media
       7.1 Tryptone salt diluent
       7.2 Special diluent (SD)
       7.3 Demineralized or distilled water
       7.4 Culture medium: MUG/EC medium
    8 Procedure
       8.1 Sample preparation
       8.2 Analysis
    9 Expression of results
       9.1 Determination of the characteristic number
       9.2 Calculation of the MPN and its confidence interval
    10 Test report
    11 Performance data
    Annex A (informative) MPN Statistical Table
    6 dilutions (1/20 to 1/2000000)/16 wells seeded per dilution
    Annex B (informative) Synthetic sea salt
    Annex C (informative) Performance data of the method
    Annex D (normative) Quality criteria for the manufacturing of
            the medium in microtitre plates (E.coli)
    Bibliography

    Abstract - (Show below) - (Hide below)

    Specifies a miniaturized most probable number (MPN) method for the quantitative detection of Escherichia coli in sludges, soils, soil improvers, growing media and biowastes. It is suitable to evaluate the log reduction of E. coli through treatment as well as the quality of the end product.

    General Product Information - (Show below) - (Hide below)

    Document Type Standard
    Publisher National Standards Authority of Ireland
    Status Current

    Standards Referencing This Book - (Show below) - (Hide below)

    ISO 5667-13:2011 Water quality Sampling Part 13: Guidance on sampling of sludges
    EN 12880:2000 Characterization of sludges - Determination of dry residue and water content
    ISO 8199:2005 Water quality General guidance on the enumeration of micro-organisms by culture
    EN ISO 5667-13:2011 Water quality - Sampling - Part 13: Guidance on sampling of sludges (ISO 5667-13:2011)
    EN ISO 9308-3:1998/AC:2000 WATER QUALITY - DETECTION AND ENUMERATION OF ESCHERICHIA COLI AND COLIFORM BACTERIA - PART 3: MINIATURIZED METHOD (MOST PROBABLE NUMBER) BY INOCULATION IN LIQUID MEDIUM
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