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I.S. EN ISO 17601:2018

Current
Current

The latest, up-to-date edition.

SOIL QUALITY - ESTIMATION OF ABUNDANCE OF SELECTED MICROBIAL GENE SEQUENCES BY QUANTITATIVE PCR FROM DNA DIRECTLY EXTRACTED FROM SOIL (ISO 17601:2016)
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

01-01-2018

Preview

For Harmonized Standards, check the EU site to confirm that the Standard is cited in the Official Journal.

Only cited Standards give presumption of conformance to New Approach Directives/Regulations.

National Foreword
European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test materials
6 Apparatus
7 Procedure
8 Examination of the critical steps of the [q]PCR assay
9 Expression of the results of the [q]PCR assay
10 International ring test
11 Test report
Annex A (informative) - Description of principal steps of
        TaqMan[R] [q]PCR assay
Annex B (informative) - International ring-test for evaluating
        [q]PCR to quantify the abundance of selected microbial
        gene sequences from DNA directly extracted from soil
Bibliography

Defines the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.

DocumentType
Standard
Pages
48
PublisherName
National Standards Authority of Ireland
Status
Current

Standards Relationship
ISO 17601:2016 Identical
EN ISO 17601:2018 Identical

ISO 10381-6:2009 Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 11063:2012 Soil quality Method to directly extract DNA from soil samples

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