Water quality Evaluation of genotoxicity by measurement of the induction of micronuclei Part 2: Mixed population method using the cell line V79

Amended by

ISO 21427-2:2006/Cor 1:2009

Available format(s)

Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users

Published date

08-11-2006

Publisher

International Organization for Standardization

ISO 21427-2:2006 specifies a method for the determination of genotoxicity of water and waste water using a mammalian in vitro test which detects damage, induced by water-soluble substances, to the chromosomes or the mitotic apparatus of V79 cells from the Chinese hamster.

The micronucleus test allows the identification of substances that cause cytogenetic damage which results in the formation of micronuclei containing lagging chromosome fragments and/or whole chromosomes.

The assay is based on the increase in the frequency of micronucleated cells after incubation with and without metabolic activation.

DevelopmentNote	Supersedes ISO/DIS 21427-2. (11/2006)
DocumentType	Standard
Pages	22
PublisherName	International Organization for Standardization
Status	Current

Standards	Relationship
I.S. EN ISO 21427-2:2009&AC:2009	Identical
NBN EN ISO 21427-2 : 2009 COR 2009	Identical
NF EN ISO 21427-2 : 2009	Identical
NEN ISO 21427-2 : 2006	Identical
NEN EN ISO 21427-2 : 2009 C1 2009	Identical
NS EN ISO 21427-2 : 2009 AC 2009	Identical
I.S. EN ISO 21427-2:2009	Identical
PN EN ISO 21427-2 : 2009	Identical
SN EN ISO 21427-2 : 2009 CORR 2009	Identical
UNI EN ISO 21427-2 : 2009	Identical
BS EN ISO 21427-2:2009	Identical
BS 6068-5.45(2006) : 2006	Identical
UNE-EN ISO 21427-2:2009	Identical
EN ISO 21427-2:2009/AC:2009	Identical
DIN EN ISO 21427-2:2009-08	Identical
EN ISO 21427-2:2009	Identical
SN EN ISO 21427-2:2009	Identical

VDI 4230 Blatt 5:2017-04 (Draft)	Biological procedures to determine environmental impact (bioindication) - Fishes as effect indicators
BS ISO 11350:2012	Water quality. Determination of the genotoxicity of water and waste water. Salmonella/microsome fluctuation test (Ames fluctuation test)
10/30197305 DC : 0	BS ISO 11350 - WATER QUALITY - DETERMINATION OF THE GENOTOXICITY OF WATER AND WASTE WATER - SALMONELA/MICROSOME FLUCTUATION TEST (AMES FLUCTUATION TEST)
ISO 11350:2012	Water quality — Determination of the genotoxicity of water and waste water — Salmonella/microsome fluctuation test (Ames fluctuation test)
17/30302216 DC : 0	BS ISO 19040-3 - WATER QUALITY - DETERMINATION OF THE ESTROGENIC POTENTIAL OF WATER AND WASTE WATER - PART 3: IN VITRO HUMAN CELL-BASED REPORTER GENE ASSAY

ISO 13829:2000	Water quality Determination of the genotoxicity of water and waste water using the umu-test
ISO 5667-10:1992	Water quality Sampling Part 10: Guidance on sampling of waste waters
ISO 5667-2:1991	Water quality Sampling Part 2: Guidance on sampling techniques
ISO 5667-14:2014	Water quality Sampling Part 14: Guidance on quality assurance and quality control of environmental water sampling and handling
ISO 5667-3:2012	Water quality Sampling Part 3: Preservation and handling of water samples
ISO 5667-1:2006	Water quality Sampling Part 1: Guidance on the design of sampling programmes and sampling techniques
ISO 16240:2005	Water quality Determination of the genotoxicity of water and waste water Salmonella/microsome test (Ames test)
ISO/TS 20281:2006	Water quality — Guidance on statistical interpretation of ecotoxicity data
ISO 5667-16:2017	Water quality — Sampling — Part 16: Guidance on biotesting of samples

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ISO 21427-2:2006

Water quality Evaluation of genotoxicity by measurement of the induction of micronuclei Part 2: Mixed population method using the cell line V79

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